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Within 3 hours the cut ends resealed, and if the segments were \u2265 \u2010 2.5 cm long they regained normal resting membrane potentials (i.e., negative to \u221280 mV). Miniture endplate potentials, endplate potentials, action potentials, the current\u2010voltage relationship, and the resting intracellular Ca<jats:sup>2+<\/jats:sup> concentration of the resealed fiber segments were similar to those in fibers that were intact from tendon to tenon. In addition, specific properties of intact fibers obtained from patients with various neuromuscular diseases were preserved in the resealed fiber segments prepared from the same patients or patients with the same diseases. These segments are easily obtained as a routine muscle biopsy performed under local anesthesia; they provide valuable preparations for the study of the pathophysiology of human skeletal muscle as well as for in vitro pharmacological tests.<\/jats:p>","DOI":"10.1002\/mus.880130309","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T09:35:32Z","timestamp":1104572132000},"page":"222-231","source":"Crossref","is-referenced-by-count":23,"title":["Resealed fiber segments for the study of the pathophysiology of human skeletal muscle"],"prefix":"10.1002","volume":"13","author":[{"given":"Frank","family":"Lehmann\u2010Horn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul A.","family":"Iaizzo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02172193"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1956.sp005502"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1984.sp015222"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1977.sp011958"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1979.sp012657"},{"key":"e_1_2_1_7_2","volume-title":"Untersuchungen \u00fcber thierische Elektrizit\u00e4t","author":"Du Bois\u2010Raymond E","year":"1848"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/46.8.603"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1960.sp006599"},{"key":"e_1_2_1_10_2","unstructured":"FrankeCh HattH IaizzoPA Lehmann\u2010HornF:Characteristics of Na+channels and Cl\u2010 conductance in resealed muscle fibre segments from patients with myotonic dystrophy.J Physiol to be published."},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880090211"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1979.sp012784"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1984.sp015317"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1973.sp010088"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00580861"},{"key":"e_1_2_1_16_2","article-title":"Long cut muscle fibers repolarize and possess electrical and mechanical properties of intact human skeletal muscle","volume":"411","author":"Iaizzo PA","year":"1988","journal-title":"Pfl\u00fcgers Arch"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880130311"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880070110"},{"key":"e_1_2_1_19_2","first-page":"44","volume-title":"Myelopathies, neuropathies et myopathies","author":"Lehmann\u2010Horn F","year":"1989"},{"key":"e_1_2_1_20_2","unstructured":"Lehmann\u2010HornF IaizzoPA:Are myotonias and periodic paralyses associated with susceptibility to malignant hyperthermia?Br J Anaesth to be published."},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","unstructured":"Lehmann\u2010HornF IaizzoPA FrankeC HattH SpaansF:Schwartz\u2010Jampel syndrome. 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