{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T15:37:43Z","timestamp":1698161863046},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,10,13]],"date-time":"2004-10-13T00:00:00Z","timestamp":1097625600000},"content-version":"vor","delay-in-days":4638,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[1992,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The purpose of this study was to determine the concentration of Ca<jats:sup>2+<\/jats:sup>\u2013ATPase and Na<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup>\u2013ATPase in biopsies from vastus lateralis muscle of 24 patients, who underwent a diagnostic contracture test for susceptibility to malignant hyperthermia (MH). Ca<jats:sup>2+<\/jats:sup>\u2013ATPase was quantified as the Ca<jats:sup>2+<\/jats:sup>\u2010dependent <jats:sup>32<\/jats:sup>P incorporation in whole muscle homogenates. Na<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup>\u2013ATPase was quantified as the [<jats:sup>3<\/jats:sup>H]ouabain\u2010binding capacity in intact muscle samples. These methods avoid isolation of membranes, a procedure that may influence the results due to interindividual variation in recovery. The results show that both enzymes can be determined in (frozen) muscle biopsies weighing 50 mg. Neither the concentration of Ca<jats:sup>2+<\/jats:sup>\u2013ATPase nor that of Na<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup>\u2013ATPase differed in biopsies from subjects diagnosed as susceptible (MHS) or nonsusceptible (MHN) to MH. Our data support the view that changes in the concentration of Ca<jats:sup>2+<\/jats:sup>\u2013ATPase and\/or Na<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup>\u2013ATPase do not play a primary role in the pathogenesis of MH.<\/jats:p>","DOI":"10.1002\/mus.880150206","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T10:35:57Z","timestamp":1104575757000},"page":"162-167","source":"Crossref","is-referenced-by-count":8,"title":["Ca<sup>2+<\/sup>\u2014ATPase and Na<sup>+<\/sup>\u2010K<sup>+<\/sup>\u2013ATPase content in skeletal muscle from malignant hyperthermia patients"],"prefix":"10.1002","volume":"15","author":[{"given":"Maria 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