{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T15:36:24Z","timestamp":1758814584949},"reference-count":12,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,12,11]],"date-time":"2005-12-11T00:00:00Z","timestamp":1134259200000},"content-version":"vor","delay-in-days":4727,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bladder outlet obstruction induces marked morphological, functional, and metabolic changes within the urinary bladder. Recent studies indicate that there is a close correlation between the contractile dysfunction induced by partial outlet obstruction and a marked decrease in mitochondrial oxidative activity of the hypertrophied bladder tissue. The current study investigates the effect of partial outlet obstruction on adenine metabolism within the bladder tissue.<\/jats:p><jats:p>After transport into the cell, adenine becomes available as a substrate for adenine phosphoribosyl transferase (APRT), the enzyme that catalyses the non\u2010mitochondrial conversion of adenine into AMP. Subsequently, AMP is phosphorylated to ADP, the phosphate acceptor in mitochondrial oxidative phosphorylation.<\/jats:p><jats:p>The results of these studies demonstrate that partial outlet obstruction induces a significant increase in <jats:sup>14<\/jats:sup>C\u2010adenine uptake into the urinary bladder smooth muscle which in turn provides substrate for APRT and results in an increase in <jats:sup>14<\/jats:sup>C\u2010AMP synthesis. In contrast, the rate of incorporation of adenine into ATP + ADP was similar for both control and obstructed tissue. The activity of APRT was not significantly different in control and obstructed tissue.<\/jats:p>","DOI":"10.1002\/nau.1930120505","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T12:06:59Z","timestamp":1173010019000},"page":"473-479","source":"Crossref","is-referenced-by-count":6,"title":["Effect of partial urinary outlet obstruction in the rabbit on the incorporation of adenine into adenine nucleotides in bladder smooth muscle"],"prefix":"10.1002","volume":"12","author":[{"given":"Niels","family":"Haugaard","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beth A. W.","family":"McKenna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan J.","family":"Wein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert M.","family":"Levin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.1930110306"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)37580-8"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)40038-3"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)40114-5"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.1930040109"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.2990170503"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000138791"},{"key":"e_1_2_1_9_1","first-page":"406","article-title":"Energetics of detrusor contraction\u2014Effects of outlet obstruction","volume":"10","author":"Lin AT\u2010L","year":"1991","journal-title":"Neurourol Urodyn"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)45331-6"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)36544-8"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.40.070171.004115"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00219316"}],"container-title":["Neurourology and Urodynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.1930120505","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.1930120505","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T19:16:49Z","timestamp":1700507809000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/nau.1930120505"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,1]]},"references-count":12,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1993,1]]}},"alternative-id":["10.1002\/nau.1930120505"],"URL":"https:\/\/doi.org\/10.1002\/nau.1930120505","archive":["Portico"],"relation":{},"ISSN":["0733-2467","1520-6777"],"issn-type":[{"value":"0733-2467","type":"print"},{"value":"1520-6777","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,1]]}}}