{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T10:27:41Z","timestamp":1782728861548,"version":"3.54.5"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,2]],"date-time":"2005-11-02T00:00:00Z","timestamp":1130889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Obstruction of the urinary bladder outlet induces detrusor smooth muscle (DSM) hypertrophy. The goal of this study was to determine whether the composition of thin filament\u2010associated proteins, known to play important roles in cytoskeletal structure and\/or the regulation of contraction, is altered in DSM during hypertrophy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>DSM hypertrophy was induced in male rabbits by partial ligation of the urethra. Sham\u2010operated rabbits served as a control. Reverse transcriptase\u2010polymerase chain reaction (RT\u2010PCR) and real\u2010time PCR revealed a significant increase in the expression of mRNAs for basic (<jats:italic>h<\/jats:italic>1) calponin (CaP), and \u03b1\u2010isoform of tropomyosin (Tm) in hypertrophied DSM compared to controls. Western blotting and two\u2010dimensional (2\u2010D) gel electrophoresis showed enhanced expression of these proteins and also a significant increase in the expression of \u03b2\u2010non muscle and \u03b3\u2010smooth muscle actin in the DSM from obstructed bladders, while \u03b1\u2010actin remained constant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Enhanced expression of these proteins in the DSM from obstructed bladders was confirmed by immunofluorescence microscopy. Double immunostaining with Cap\/Tm and \u03b1\/\u03b2\u2010actin\u2010specific antibodies showed co\u2010localization of these proteins in myocytes. Colocalization of smooth muscle specific myosin and CaP to cytoplasmic filaments in cells dissociated from the hypertrophied DSM indicated that these cells are differentiated smooth muscle cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The change in the isoforms of actin, Cap, and Tm may be part of the molecular mechanism for bladder compensation in increased urethral resistance. Neurourol. 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