{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:19:11Z","timestamp":1762100351310},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,3,24]],"date-time":"2005-03-24T00:00:00Z","timestamp":1111622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Spontaneously hypertensive rats (SHR) exhibit overactive bladder (OAB) symptoms and have an up\u2010regulated calcium sensitizing RhoA\/Rho\u2010kinase pathway in their vascular smooth muscle tissues. This study examined the role of RhoA\/Rho\u2010kinase pathway in bladder hyperactivity by evaluating the effect of a specific Rho\u2010kinase inhibitor (Y\u201027632) on SHR bladder function.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Adult male SHR (n\u2009=\u20099) and their normotensive controls (Wistar\u2013Kyoto; WKY) (n\u2009=\u20098) were anesthetized and the carotid artery cannulated for blood pressure monitoring. A catheter was fixed into the bladder dome and connected to a pressure transducer and an infusion pump. After equilibration, systemic and bladder pressure were recorded. Continuous filling cystometrograms (CMGs) were performed and threshold pressure (TP), peak pressure (PP), and number of voids and non\u2010voiding contractions (NVCs) per unit time recorded. Each SHR then received Y\u201027632, 10 \u03bcmol intra\u2010arterially. After 10 min, CMG was repeated and the same measurements recorded. Bladder tissues were evaluated immunohistochemically (IHC) for RhoA protein expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SHR exhibited significantly higher number of voids and NVCs than normotensive WKY rats (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). In SHR, Y\u201027362 administration significantly decreased the number of voids (29%, from 0.83\u2009\u00b1\u20090.3 to 0.63\u2009\u00b1\u20090.17 voids\/min) and NVCs (61%, from 1.8\u2009\u00b1\u20090.54 to 0.64\u2009\u00b1\u20090.167 NVC\/min). IHC showed significantly higher RhoA protein expression in SHR bladder tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Overexpression of RhoA may play a role in hypertension\u2010related OAB. Inhibition of Rho\u2010kinase activity with Y\u201027632 produced a significant suppression of bladder overactivity. Identification of Rho\u2010kinase isoforms that are bladder\u2010tissue specific and their selective inhibitors may help to disassociate the unwanted hypotensive effects of this approach. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.20122","type":"journal-article","created":{"date-parts":[[2005,3,24]],"date-time":"2005-03-24T22:46:48Z","timestamp":1111704408000},"page":"295-300","source":"Crossref","is-referenced-by-count":66,"title":["Rho\u2010kinase inhibition suppresses bladder hyperactivity in spontaneously hypertensive rats"],"prefix":"10.1002","volume":"24","author":[{"given":"Mahadevan","family":"Rajasekaran","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan","family":"Wilkes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Kuntz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"E. 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