{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T15:53:20Z","timestamp":1761580400618,"version":"3.32.0"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,5,23]],"date-time":"2006-05-23T00:00:00Z","timestamp":1148342400000},"content-version":"vor","delay-in-days":142,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate the role played by pituitary adenylate cyclase activating polypeptide 38 (PACAP 38) in the non\u2010adrenergic non\u2010cholinergic (NANC) neurotransmission of the pig urinary bladder neck.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Urothelium\u2010denuded bladder neck strips were dissected and mounted in organ baths containing a physiological saline solution (PSS) at 37\u00b0C and gassed with 5% CO<jats:sub>2<\/jats:sub>and 95% O<jats:sub>2<\/jats:sub>, for isometric force recording. The relaxations to transmural nerve stimulation (EFS) or PACAP 38 were performed on strips precontracted with 1 \u00b5M phenylephrine (PhE). EFS experiments were carried out in the absence and the presence of guanethidine (10 \u00b5M), atropine (0.1 \u00b5M), and N<jats:sup>G<\/jats:sup>\u2010nitro\u2010<jats:sc>l<\/jats:sc>\u2010arginine (L\u2010NOARG, 100 \u00b5M), to block noradrenergic neurotransmission, muscarinic receptors, and nitric oxide (NO) synthase, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>EFS (2\u201316 Hz, 1 ms duration, 20 sec trains, 75 mA current output) evoked frequency\u2010dependent relaxations which were reduced by the VIP\/PACAP receptor antagonist PACAP (6\u201338) (3 \u00b5M), and by the neurotoxin of the capsaicin\u2010sensitive primary afferents capsaicin (10 \u00b5M), and abolished by the neuronal voltage\u2010activated Na<jats:sup>+<\/jats:sup>channel blocker tetrodotoxin (TTX, 1 \u00b5M). The vasoactive intestinal peptide (VIP) receptor antagonist [Lys<jats:sup>1<\/jats:sup>, Pro<jats:sup>2,5<\/jats:sup>, Arg<jats:sup>3,4<\/jats:sup>, Tyr<jats:sup>6<\/jats:sup>]\u2010VIP (3 \u00b5M) failed to modify the EFS\u2010induced relaxations. PACAP 38 (1 nM\u20131 \u00b5M) induced concentration\u2010dependent relaxations which were reduced by PACAP (6\u201338), TTX and by the neuronal voltage\u2010gated Ca<jats:sup>2+<\/jats:sup>channel inhibitor \u03c9\u2010conotoxin GVIA (\u03c9\u2010CgTX, 1 \u00b5M).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results suggest that PACAP 38, mainly released from capsaicin\u2010sensitive primary afferents, is involved in the NANC inhibitory neurotransmission of the pig urinary bladder neck, producing relaxation through neuronal and muscle VIP\/PACAP receptor activation. Neurourol. Urodynam. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.20287","type":"journal-article","created":{"date-parts":[[2006,5,23]],"date-time":"2006-05-23T23:01:03Z","timestamp":1148425263000},"page":"490-497","source":"Crossref","is-referenced-by-count":20,"title":["PACAP 38 is involved in the non\u2010adrenergic non\u2010cholinergic inhibitory neurotransmission in the pig urinary bladder neck"],"prefix":"10.1002","volume":"25","author":[{"given":"Medardo","family":"Hern\u00e1ndez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Victoria","family":"Barahona","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paz","family":"Recio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvador","family":"Bustamante","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Benedito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Rivera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albino","family":"Garc\u00eda\u2010Sacrist\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dolores","family":"Prieto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis M.","family":"Orensanz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,23]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)52361-7"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1995.tb00765.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)67201-1"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.1007"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1998.275.4.G769"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(97)00474-0"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1838(94)90005-1"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410050980"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0031-6997(24)01360-7","article-title":"International Union of Pharmacology. 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