{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T02:46:56Z","timestamp":1773456416997,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,1,31]],"date-time":"2007-01-31T00:00:00Z","timestamp":1170201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Vanilloids like capsaicin and resiniferatoxin (RTX) have been used for more than a decade in the treatment of neurogenic detrusor overactivity. Recently, the vanilloid molecule piperine (PIP) has been shown to have similar pharmacological properties as these drugs. In this study, we looked at PIP\u2010effects on autonomous bladder contractile activity, with particular interest for its selectivity for the transient receptor potential channel 1 (TRPV1) receptor. Additionally, we studied the role of TRPV1 in volume\u2010induced contractile changes using selective and non\u2010selective TRPV1 antagonists.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The acute and prolonged effects of PIP were studied on rat bladders. Each bladder was excised and placed in a heated organ bath, where intravesical pressures were measured. In acute experiments, PIP was added directly to the bathing solution. For prolonged effects, animals were pre\u2010treated intravesically with vehicle (ethanol 5%) or PIP (10<jats:sup>\u22124<\/jats:sup>M) and sacrificed 72 hr later. The effects of selective (capsazepine (CZP)) and non\u2010selective (ruthenium red (RR)) TRPV1 antagonists on volume\u2010evoked contractile parameters were also studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Acute administration of PIP 10<jats:sup>\u22124<\/jats:sup>M significantly increased amplitude of bladder contractions (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). These effects were significantly antagonized (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) by the TRPV1\u2010selective antagonist CZP (10<jats:sup>\u22125<\/jats:sup>M) and the non\u2010selective TRP\u2010antagonist RR (10<jats:sup>\u22125<\/jats:sup>M). Intravesical pre\u2010treatment with PIP induced shorter contractions with more periods of non\u2010activity (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) compared to controls. Inhibition of TRPV1 with CZP and RR significantly reduced the volume\u2010evoked rise in contractile amplitude in isolated bladders (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We found evidence for acute and prolonged effects of PIP on bladder contractility, which seem to be mediated through TRPV1. Furthermore, we found evidence for involvement of TRPV1 in afferent signaling of mechanical stimuli. Neurourol. Urodynam. 26:440\u2013450, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.20343","type":"journal-article","created":{"date-parts":[[2007,2,3]],"date-time":"2007-02-03T05:33:41Z","timestamp":1170480821000},"page":"440-450","source":"Crossref","is-referenced-by-count":34,"title":["TRPV1 is involved in stretch\u2010evoked contractile changes in the rat autonomous bladder model: a study with piperine, a new TRPV1 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