{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T12:46:54Z","timestamp":1758631614530},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,1,31]],"date-time":"2007-01-31T00:00:00Z","timestamp":1170201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate the nitric oxide (NO)\u2010mediated nerve relaxation and its possible modulation by pre\u2010junctional \u03b1<jats:sub>2<\/jats:sub>\u2010adrenoceptors in the pig urinary bladder neck.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Urothelium\u2010denuded bladder neck strips were dissected, and mounted in isolated organ baths containing a physiological saline solution (PSS) at 37\u00b0C and continuously gassed with 5% CO<jats:sub>2<\/jats:sub> and 95% O<jats:sub>2<\/jats:sub>, for isometric force recording. The relaxations to transmural nerve stimulation (electrical field stimulation [EFS]) or exogenously applied NO were carried out on strips pre\u2010contracted with 1 \u00b5M phenylephrine (PhE) and treated with guanethidine (10 \u00b5M) and atropine (0.1 \u00b5M), to block noradrenergic neurotransmission and muscarinic receptors, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>EFS (0.2\u20131 Hz, 1 msec duration, 20 sec trains, current output adjusted to 75 mA) evoked frequency\u2010dependent relaxations which were abolished by the neuronal voltage\u2010activated Na<jats:sup>+<\/jats:sup> channel blocker tetrodotoxin (TTX, 1 \u00b5M). These responses were potently reduced by the nitric oxide synthase (NOS) inhibitor N<jats:sup>G<\/jats:sup>\u2010nitro\u2010<jats:sc>L<\/jats:sc>\u2010arginine (L\u2010NOARG, 30 \u00b5M) and further reversed by the NO synthesis substrate <jats:sc>L<\/jats:sc>\u2010arginine (L\u2010ARG, 3 mM). The \u03b1<jats:sub>2<\/jats:sub>\u2010adrenoceptor agonist BHT\u2010920 (2 \u00b5M) reduced the electrically evoked relaxations, its effectiveness being higher on the responses induced by low frequency stimulation. BHT\u2010920\u2010elicited reductions were fully reversed by the \u03b1<jats:sub>2<\/jats:sub>\u2010adrenoceptor antagonist rauwolscine (RAW, 1 \u00b5M). Exogenous NO (1 \u00b5M\u20131 mM) induced concentration\u2010dependent relaxations which were not modified by BHT\u2010920, thus eliminating a possible post\u2010junctional modulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results indicate that NO is involved in the non\u2010adrenergic non\u2010cholinergic (NANC) inhibitory neurotransmission in the pig urinary bladder neck, the release of NO from intramural nerves being modulated by pre\u2010junctional \u03b1<jats:sub>2<\/jats:sub>\u2010adrenoceptor stimulation. Neurourol. Urodynam. 26:578\u2013583, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.20368","type":"journal-article","created":{"date-parts":[[2007,2,3]],"date-time":"2007-02-03T05:07:02Z","timestamp":1170479222000},"page":"578-583","source":"Crossref","is-referenced-by-count":19,"title":["Pre\u2010junctional \u03b1<sub>2<\/sub>\u2010adrenoceptors modulation of the nitrergic transmission in the pig urinary bladder neck"],"prefix":"10.1002","volume":"26","author":[{"given":"Medardo","family":"Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"Departamento de Fisiolog\u00eda (Fisiolog\u00eda Animal) Facultad de Farmacia Universidad Complutense de Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paz","family":"Recio","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda (Fisiolog\u00eda Animal) Facultad de Farmacia Universidad Complutense de Madrid  Madrid 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