{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T11:57:11Z","timestamp":1784030231016,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,2,5]],"date-time":"2007-02-05T00:00:00Z","timestamp":1170633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate the activity of the T\u2010type calcium channel (TCC) and the expression of its subtypes in overactive detrusor (OD) myocytes in rats after partial bladder outflow obstruction (PBOO).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirteen male Wistar rats with OD after PBOO (OD group) and eight sham\u2010operated rats (control group) were studied. The two groups were compared regarding the expression of TCC subtype genes by reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) and the TCC kinetics and cell action potential by whole\u2010cell patch\u2010clamp.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The time course and density of the current were significantly higher in the OD cells than those in the control detrusor. Whole\u2010cell patch\u2010clamp analysis showed that the activation of TCCs in detrusor myocytes in the OD group was faster than the control group, but inactivation was almost the same in both groups, suggesting a significant enhancement of the Ca<jats:sup>2+<\/jats:sup> \u201cwindow\u201d current in the OD group. Patch\u2010clamp recording of action potentials in the OD cells indicated an increase in excitability and a decrease in the repolarization interval. RT\u2010PCR assay showed an abnormal expression of \u03b11G subtype in the OD cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>TCCs could be one of the crucial factors for the abnormal excitation in OD cells. The development of OD after PBOO presumably relates to the increase in TCC current in the bladder cells, the enhancement of the Ca<jats:sup>2+<\/jats:sup> \u201cwindow\u201d current for Ca<jats:sup>2+<\/jats:sup> inflow, the prolongation of the intracellular calcium oscillations, and the acceleration of the cell depolarization. Neurourol. Urodynam. 26:870\u2013878, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.20392","type":"journal-article","created":{"date-parts":[[2007,2,5]],"date-time":"2007-02-05T22:15:51Z","timestamp":1170713751000},"page":"870-878","source":"Crossref","is-referenced-by-count":19,"title":["Changes in T\u2010type calcium channel and its subtypes in overactive detrusor of the rats with partial bladder outflow 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