{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T16:59:01Z","timestamp":1759683541605},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,3,23]],"date-time":"2007-03-23T00:00:00Z","timestamp":1174608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Chronic pelvic pain disorders often overlap. We have shown that acute colonic irritation can produce acute irritative micturition patterns and acutely sensitize bladder afferent responses to mechanical and chemical stimuli. We hypothesize that with time, colonic irritation can lead to neurogenic changes in the bladder and the development of chronic bladder sensitization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Micturition patterns were measured in rats 60\u201390 days after the induction of trinitrobenzenesulfonic acid (TNBS) colitis in the resolution phase of this model. Total and activated mast cells (MCs) were quantified in the bladder, while mRNA levels of stem cell factor (SCF; a.k.a. MC growth factor) and nerve growth factor (NGF; a MC and nociceptive C\u2010fiber stimulator) were quantified in the bladder and L6\u2010S1 dorsal root ganglia (DRG).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Following intra\u2010rectal TNBS, voiding volume was reduced (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.005), while voiding frequency was increased (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), both by \u223c50%. Furthermore, both the percentage and density of activated bladder MCs were significantly elevated (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), although total MC counts were not statistically increased. At the molecular level, urinary bladder SCF expression increased twofold (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.005), as did NGF (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), while L6\u2010S1 DRG levels were not significantly elevated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Chronic cystitis in the rat as evidenced by changes in micturition patterns and the recruitment of activated MCs can occur during the resolution phase of TNBS colitis. These changes, of which MCs may play an important role, appear to be maintained over time and may occur via stimulation of convergent pelvic afferent input resulting in the upregulation of neurotrophic factors in the target organ. Neurourol. Urodynam. 26:887\u2013893, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.20410","type":"journal-article","created":{"date-parts":[[2007,3,23]],"date-time":"2007-03-23T17:03:34Z","timestamp":1174669414000},"page":"887-893","source":"Crossref","is-referenced-by-count":19,"title":["Enhanced expression of mast cell growth factor and mast cell activation in the bladder following the resolution of trinitrobenzenesulfonic acid (TNBS) colitis in female rats"],"prefix":"10.1002","volume":"26","author":[{"given":"Ruomei","family":"Liang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena E.","family":"Ustinova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radhika","family":"Patnam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew 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