{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:23:58Z","timestamp":1773419038586,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,8,27]],"date-time":"2007-08-27T00:00:00Z","timestamp":1188172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Japanese Ministry of Education, Science, Sports and Culture","doi-asserted-by":"crossref","award":["17791085"],"award-info":[{"award-number":["17791085"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Japanese Ministry of Education, Science, Sports and Culture","doi-asserted-by":"crossref","award":["19791117"],"award-info":[{"award-number":["19791117"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Japanese Ministry of Education, Science, Sports and Culture","doi-asserted-by":"crossref","award":["17390443"],"award-info":[{"award-number":["17390443"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>We investigated the effects of bladder outlet obstruction (BOO) on the distribution of interstitial cells (ICs) in the guinea\u2010pig bladder.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bladder overactivity of BOO animals was validated with urodynamic studies. Immunohistochemical analyses for Kit and vimentin as markers for ICs were performed on both BOO and control bladders. Morphological and functional properties of detrusor smooth muscle (DSM) were examined with \u03b1\u2010smooth muscle actin staining and intracellular recording, respectively. Electron microscopy was also carried out to characterize ultrastructural morphology of ICs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two weeks after surgery, BOO animals showed an increased voiding frequency and a reduced voiding volume. Filling cystometry demonstrated a frequent incidence of non\u2010voiding contractions, a reduced interval between voiding contractions and an increased voiding pressure in BOO bladders. In BOO bladders, the thickness of suburothelial and subserosal connective tissue layers was increased, whilst that of detrusor smooth muscle (DSM) layer was less affected. Population of Kit or vimentin immunoreactive ICs was increased in subserosal layers, and their distribution was altered in suburotherial layer in BOO bladders. Neither \u03b1\u2010actin immunoreactivity nor spontaneous electrical activity of DSM was altered in BOO bladders. ICs were characterized by their numerous mitochondria and caveolae, and had a close contact with each other and with neighboring DSM or nerves.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results demonstrated the increased population of ICs in the BOO guinea\u2010pig model for the first time, and suggest that the altered distribution of ICs may contribute to the pathophysiology of bladder overactivity. Neurourol. Urodynam. 27:330\u2013340, 2008. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.20502","type":"journal-article","created":{"date-parts":[[2007,8,27]],"date-time":"2007-08-27T18:16:56Z","timestamp":1188238616000},"page":"330-340","source":"Crossref","is-referenced-by-count":63,"title":["Altered distribution of interstitial cells in the guinea pig bladder following bladder outlet obstruction"],"prefix":"10.1002","volume":"27","author":[{"given":"Yasue","family":"Kubota","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hikaru","family":"Hashitani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuyuki","family":"Shirasawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiyuki","family":"Kojima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoichi","family":"Sasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshio","family":"Mabuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuyoshi","family":"Soji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hikaru","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenjiro","family":"Kohri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,8,27]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)32614-9"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(97)00591-8"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(97)00587-6"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1996.v111.pm8690216"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.043299"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.116855"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.116632"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000146272.80848.37"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1464-410X.2002.02834.x"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)64752-0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(95)00453-X"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705602"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000069722.35137.e0"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)05549-0"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2005.05988.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.9.2.409"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.62.295"},{"key":"e_1_2_1_19_2","first-page":"F885","article-title":"Changes in electrical properties of guinea pig smooth muscle membrane by experimental bladder outflow obstruction","volume":"262","author":"Seki N","year":"1992","journal-title":"Am J Physiol"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00294493"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0273l.x"},{"key":"e_1_2_1_22_2","doi-asserted-by":"crossref","first-page":"2002","DOI":"10.1016\/S0022-5347(05)67618-5","article-title":"Distribution of P2X receptors in the urinary bladder and the ureter of the rat","volume":"162","author":"Lee HY","year":"2000","journal-title":"J Urol"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/35039519"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2004.05186.x"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20085"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.281.3.G602"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.t01-2-00359.x"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0867n.x"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2006.06617.x"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000054928.34777.37"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)37192-6"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.058628"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.065136"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0555c.xd"}],"container-title":["Neurourology and Urodynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.20502","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.20502","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.20502","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T01:36:22Z","timestamp":1700530582000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/nau.20502"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8,27]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,4]]}},"alternative-id":["10.1002\/nau.20502"],"URL":"https:\/\/doi.org\/10.1002\/nau.20502","archive":["Portico"],"relation":{},"ISSN":["0733-2467","1520-6777"],"issn-type":[{"value":"0733-2467","type":"print"},{"value":"1520-6777","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8,27]]}}}