{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T18:54:24Z","timestamp":1775674464870,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,3,12]],"date-time":"2009-03-12T00:00:00Z","timestamp":1236816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate possible mechanisms of action of THC\u2010002 (HARNCARE\u00ae), a galenical produced from the traditional Chinese herbal mixture Ba\u2010Wei\u2010Die\u2010Huang\u2010Wan, which has been reported to improve lower urinary tract symptoms (LUTS) in patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Forty\u2010five female SHRs were randomly separated into three groups. Two groups were given 20 ml physiological saline solution (PSS) per kg\u2010body weight orally daily for 1 week. An hour after the administration of PSS, one of the groups received 20 mg THC\u2010002 per kg body weight, and the other a similar volume of THC\u2010002\u2010free saline. The third group received no treatments. The bladders were analyzed by real time RT\u2010PCR (n\u2009=\u20096) and immunohistochemistry (n\u2009=\u20093) for the expression of tachykinins and P2X3 and TRPV1 receptors. Cystometric investigation (n\u2009=\u20096) was conducted after intravesical instillation of saline followed by 5 mg\/ml ATP solution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Treatment with PSS caused and upregulation of tachykinins and P2X3 and TRPV1 receptors, which was prevented in the group treated with THC\u2010002. In the normal (non\u2010treated) and non\u2010THC\u2010002\u2010treated SHRs, instillation of the ATP solution decreased voiding interval, micturition volume, and bladder capacity compared to the instillation of saline. However, in the THC\u2010002\u2010treated SHRs, ATP instillation had no effect.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In SHRs, THC\u2010002 reduced the bladder expression of tachykinins and P2X3 and TRPV1 receptors, and inhibited ATP\u2010induced detrusor overactivity. These effects may explain part of its beneficial effects on LUTS. Neurourol. Urodyn. 28:529\u2013534, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.20702","type":"journal-article","created":{"date-parts":[[2009,3,12]],"date-time":"2009-03-12T19:33:38Z","timestamp":1236886418000},"page":"529-534","source":"Crossref","is-referenced-by-count":21,"title":["An extract (THC\u2010002) of Ba\u2010Wei\u2010Die\u2010Huang\u2010Wan inhibits expression of tachykinins, and P2X3 and TRPV1 receptors, and inhibits ATP\u2010induced detrusor overactivity in spontaneously hypertensive 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