{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T20:04:45Z","timestamp":1780344285826,"version":"3.54.1"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,9,21]],"date-time":"2009-09-21T00:00:00Z","timestamp":1253491200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To compare the mRNA expression of extracellular matrix (ECM) proteins in postmenopausal prolapsed versus non\u2010prolapsed anterior vaginal wall (AVW) tissue. We hypothesized that the weakening of the tissue leading to prolapse was due to decreased collagen production from a downregulation at the transcriptional level.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Following IRB approval, full thickness samples of redundant AVW were excised from consecutive age\u2010equivalent, postmenopausal, women undergoing cystocele repair (prolapse, stage III or IV), or radical cystectomy (control, no clinical findings of prolapse). Total RNA was isolated, cDNA was synthesized, and quantitative real\u2010time polymerase chain reaction (PCR) was conducted to assess the mRNA expression of collagens type I and III, pro\u2010elastin, MMP3, MMP10, and MMP11. The significance of the difference of mRNA expression between prolapse and control tissues was tested using Student's t\u2010test followed by Mann\u2013Whitney Rank Sum Test.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A 5.3\u2010fold increase in collagen type I mRNA was found in prolapse (n\u2009=\u200947) over control (n\u2009=\u20097) tissues (P\u2009=\u20090.009). Type III collagen mRNA was also significantly increased to a 3.3 times higher level (P\u2009=\u20090.017). The ratio of type III to type I was decreased from 15.6 in controls to 9.7 in prolapse. An increasing trend in pro\u2010elastin and MMP mRNA expression was found in prolapse, but this was not statistically significant.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In this controlled study, the increase found in collagen mRNA expression disproved our hypothesis. To the contrary, this defective prolapsed tissue can signal its need for ECM replenishment. The message, however, is not being effectively translated to assist in tissue remodeling. Neurourol. Urodynam. 29:582\u2013586, 2010. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/nau.20806","type":"journal-article","created":{"date-parts":[[2010,7,9]],"date-time":"2010-07-09T06:59:38Z","timestamp":1278658778000},"page":"582-586","source":"Crossref","is-referenced-by-count":26,"title":["Extracellular matrix expression of human prolapsed vaginal wall"],"prefix":"10.1002","volume":"29","author":[{"given":"Elizabeth","family":"Mosier","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor K.","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Zimmern","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,9,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-006-0102-1"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1997.tb11536.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20048"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003081-199006000-00013"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(99)70203-6"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.6.040803.140250"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)91489-0"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2005.00773.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0412.2003.00019.x"},{"key":"e_1_2_1_11_2","first-page":"583","article-title":"Decreased collagen synthesis in stress\u2010incontinent women","volume":"84","author":"Falconer C","year":"1994","journal-title":"Obstet Gynecol"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-006-0090-1"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001920200076"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2003.09.043"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deg420"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000182584.15087.dd"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-005-1294-5"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-004-1215-z"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-005-0045-y"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2007.06998.x"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-007-0516-4"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2008.01.027"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20657"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0029-7844(99)00336-1"},{"key":"e_1_2_1_25_2","doi-asserted-by":"crossref","first-page":"15579","DOI":"10.1016\/S0021-9258(18)98442-5","article-title":"Cleavage of cartilage proteoglycan between G1 and G2 domains by stromelysins","volume":"266","author":"Fosang AJ","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_1_26_2","first-page":"1","article-title":"Extracellular matrix expression of human prolapsed vaginal wall","volume":"28","author":"Mosier E","year":"2009","journal-title":"Neurourol Urodynam"}],"container-title":["Neurourology and Urodynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.20806","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.20806","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.20806","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T17:10:50Z","timestamp":1700500250000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/nau.20806"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,21]]},"references-count":25,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["10.1002\/nau.20806"],"URL":"https:\/\/doi.org\/10.1002\/nau.20806","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.2246957.1862055","asserted-by":"object"}]},"ISSN":["0733-2467","1520-6777"],"issn-type":[{"value":"0733-2467","type":"print"},{"value":"1520-6777","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,21]]}}}