{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:15:51Z","timestamp":1791090951378,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2010,2,11]],"date-time":"2010-02-11T00:00:00Z","timestamp":1265846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Relaxation of detrusor muscle via \u03b2\u2010adrenoceptors may contribute to urine storage during bladder filling. Thus there is increasing interest in \u03b2\u2010adrenoceptor agonists as a potential treatment for detrusor overactivity. The role of the urothelium in bladder responses to \u03b2\u2010adrenoceptor agonists is not yet clear, although we have shown that these agonists have a greater inhibitory effect on detrusor contraction when the urothelium is intact. The aim was to determine which \u03b2\u2010adrenoceptor subtype is involved in this effect.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Paired strips of pig bladder dome mucosa\u2010intact and mucosa\u2010denuded, were mounted in tissue baths. Relaxation responses were obtained to \u03b2\u2010adrenoceptor agonists (isoprenaline, dobutamine, salbutamol or BRL37344) in carbachol pre\u2010contracted tissues. Inhibitory effects were studied by obtaining concentration\u2010response curves (CRCs) to carbachol in the presence and absence of \u03b2\u2010adrenoceptor agonists. The inhibitory effects of isoprenaline were also studied following incubation with \u03b2\u2010adrenoceptor antagonists (propranolol, CGP20712, ICI\u2010118, 551 or SR 59230A; non selected, \u03b2<jats:sub>1<\/jats:sub>, \u03b2<jats:sub>2<\/jats:sub> and \u03b2<jats:sub>3<\/jats:sub> selective respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>isoprenaline, dobutamine, salbutamol and BRL37344 all relaxed carbachol pre\u2010contracted tissues and responses were similar in intact and denuded strips. In inhibition experiments, \u03b2\u2010adrenoceptor agonists caused rightward shifts of carbachol CRCs. In intact strips the shift was greater with isoprenaline and BRL37344, but not with dobutamine or salbutamol. This increased shift was still observed in tissues pre\u2010incubated with propranolo, CGP20712 and ICI\u2010118, 551, but not with SR 59230A.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>\u03b2<jats:sub>3<\/jats:sub>\u2010adrenoceptors are involved in mediating inhibitory effects of \u03b2\u2010adrenoceptor agonists on detrusor contractions via the urothelium in pig bladder dome. Neurourol. Urodynam. 29:1320\u20131325, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.20838","type":"journal-article","created":{"date-parts":[[2010,2,11]],"date-time":"2010-02-11T20:45:28Z","timestamp":1265921128000},"page":"1320-1325","source":"Crossref","is-referenced-by-count":46,"title":["The \u03b2<sub>3<\/sub>\u2010adrenoceptor mediates the inhibitory effects of \u03b2\u2010adrenoceptor agonists via the urothelium in pig bladder dome"],"prefix":"10.1002","volume":"29","author":[{"given":"Koichi","family":"Masunaga","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher R.","family":"Chapple","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil G.","family":"McKay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna J.","family":"Sellers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,9,7]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.104.077768"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(67)90031-8"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(94)90118-X"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-8673.1982.tb00517.x"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)42659-0"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/nau.1930120213"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704470"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000067621.62736.7c"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005392-200101000-00071"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00007101"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2007.01.059"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2006.06564.x"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/1478-811X-3-10"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-18-08063.2002"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2006.06679.x"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703068"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)69137-7"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000091870.51841.ae"},{"key":"e_1_2_5_20_2","first-page":"474","article-title":"Expression and functional role of \u03b2\u2010adrenoceptors in the human urinary bladder urothelium","volume":"377","author":"Otsuka A","year":"2008","journal-title":"Naunyn\u2010Scmiedeberg's Arch Pharmacol"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)67587-6"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2005.08.014"}],"container-title":["Neurourology and Urodynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.20838","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.20838","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.20838","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T22:04:44Z","timestamp":1700517884000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/nau.20838"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9]]},"references-count":21,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1002\/nau.20838"],"URL":"https:\/\/doi.org\/10.1002\/nau.20838","archive":["Portico"],"relation":{},"ISSN":["0733-2467","1520-6777"],"issn-type":[{"value":"0733-2467","type":"print"},{"value":"1520-6777","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9]]}}}