{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:34:17Z","timestamp":1785414857845,"version":"3.56.0"},"reference-count":19,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["5R01AG028657"],"award-info":[{"award-number":["5R01AG028657"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>In vivo animal cystometry represents an accepted methodology for the study of lower urinary tract physiology. A particular advantage of the mouse model is the availability of genetically modified strains, offering the possibility of linking individual genes to relevant physiological events. However, small voided volumes complicate the ability to obtain reliable pressure\u2010flow data by gravimetric methods, due to non\u2010continuous drop formation and release during voiding. We investigated the feasibility of a simple non\u2010gravimetric continuous urine collection system during cystometry under urethane anesthesia, and compared urethane\u2010anesthetized with awake cystometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cystometry was performed in awake and urethane\u2010anesthetized female mice using a suprapubic tube. A simple, novel non\u2010gravimetric method of urine collection was used in urethane\u2010anesthetized animals to assess voided volume and permit flow rate calculations. Pressure and time\u2010related variables were compared between groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Voided urine collection appears to be complete and continuous in this model. Mean voided volume was 0.09\u2009\u00b1\u20090.020\u2009ml, with an average flow rate of 0.029\u2009\u00b1\u20090.007\u2009ml\/sec. Urethane anesthesia delayed cystometric pressure\/volume responses. However, micturition reflexes were intact and otherwise comparable between groups. Female mice void with pulsatile pressurization previously described in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Suprapubic voiding cystometry using a simple and reliable urine collection method under urethane anesthesia is feasible in mice, permitting the integration of voided volumes with pressure and time data. The inclusion of volume and flow data enhances the usefulness of the mouse model for in vivo assessment of detrusor and potentially sphincteric performance. Neurourol. Urodynam. 29:1344\u20131349, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.20850","type":"journal-article","created":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T20:12:52Z","timestamp":1265055172000},"page":"1344-1349","source":"Crossref","is-referenced-by-count":41,"title":["Continuous uroflow cystometry in the urethane\u2010anesthetized mouse"],"prefix":"10.1002","volume":"29","author":[{"given":"Phillip P.","family":"Smith","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George A.","family":"Kuchel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,9,7]]},"reference":[{"key":"e_1_2_7_2_2","unstructured":"RowlettR.How Many? A Dictionary of Units of Measure. University of North Carolina at Chapel Hill; 2005 [updated 2005; cited]; Available from:http:\/\/www.unc.edu\/\u223crowlett\/units\/."},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000134186.58854.2c"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1838(90)90256-I"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01952426"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(01)01182-1"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000161210.17365.cc"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)66590-1"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)65151-6"},{"key":"e_1_2_7_10_2","unstructured":"AVMA Guidelines on Euthanasia: American Veterinary Medical Association; 2007 Contract No.: Document Number|."},{"key":"e_1_2_7_11_2","volume-title":"Laboratory animal anaesthesia: An introduction for research workers and technicians","author":"Flecknell PA","year":"1987"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-4096.2004.04875.x"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20229"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)64911-7"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2005.05811.x"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2008.10.047"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-6777(2000)19:1<87::AID-NAU9>3.0.CO;2-O"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00590037"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1999.277.5.R1368"},{"key":"e_1_2_7_20_2","first-page":"30","article-title":"Pressurization of the mid\u2010urethra activates the external urethral sphincter in rats","volume":"13","author":"Hurtado E","year":"2007","journal-title":"J Pelvic Med Surg [abstract]"}],"container-title":["Neurourology and Urodynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.20850","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.20850","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.20850","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T22:04:47Z","timestamp":1700517887000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/nau.20850"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9]]},"references-count":19,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1002\/nau.20850"],"URL":"https:\/\/doi.org\/10.1002\/nau.20850","archive":["Portico"],"relation":{},"ISSN":["0733-2467","1520-6777"],"issn-type":[{"value":"0733-2467","type":"print"},{"value":"1520-6777","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9]]}}}