{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T13:43:01Z","timestamp":1775914981700,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,4,28]],"date-time":"2010-04-28T00:00:00Z","timestamp":1272412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>The clinical indication of phenazopyridine is unclear, it has been used clinically in conditions with increased bladder sensation as in cystitis and bladder pain syndrome\/interstitial cystitis. We explored the effect of phenazopyridine on afferent nerve activity by direct measurement of both A\u03b4\u2010 and C\u2010fibers in the rat, and compared the outcome with the effects of lidocaine (a local anesthetic) and of acetaminophen (an analgesic).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Female Sprague\u2013Dawley rats were used. Under urethane anesthesia, single nerve fibers primarily originating from the bladder were identified in the L6 dorsal root by electrical stimulation of the left pelvic nerve and by bladder distention. By conduction velocity (2.5\u2009m\/sec) the fibers were defined as A\u03b4\u2010fiber or C\u2010fiber. The afferent activity in response to constant bladder filling was measured before the drug administration. Then, phenazopyridine (0.1\u20133\u2009mg\/kg) or lidocaine (0.3\u20133\u2009mg\/kg) or acetaminophen (1\u201310\u2009mg\/kg) was administrated intravenously. After drug administration, the afferent activity of bladder fillings was measured again.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All drugs significantly increased bladder compliance, in a dose\u2010dependent way. Twenty\u2010eight single afferent fibers (A\u03b4\u2010fibers: n\u2009=\u200913, C\u2010fibers: n\u2009=\u200915) were isolated. Intravenous administration of phenazopyridine significantly decreased dose\u2010dependently only the A\u03b4\u2010fiber but not the C\u2010fiber activity. Also acetaminophen significantly decreased only A\u03b4\u2010fiber activity, but it was not dose\u2010dependently completely. Lidocaine inhibited both the A\u03b4\u2010 and C\u2010fiber activities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study shows that phenazopyridine can directly inhibit the mechanosensitive A\u03b4\u2010fibers in the normal rat bladder. This finding might explain its clinical effect in conditions of bladder hypersensitivity. Neurourol. Urodynam. 29:1445\u20131450, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.20886","type":"journal-article","created":{"date-parts":[[2010,4,29]],"date-time":"2010-04-29T14:51:54Z","timestamp":1272552714000},"page":"1445-1450","source":"Crossref","is-referenced-by-count":19,"title":["Effects of phenazopyridine on rat bladder primary afferent activity, and comparison with lidocaine and 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