{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:07:07Z","timestamp":1758632827475,"version":"3.37.3"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,12,22]],"date-time":"2010-12-22T00:00:00Z","timestamp":1292976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n, Spain","doi-asserted-by":"crossref","award":["PS09\/00044"],"award-info":[{"award-number":["PS09\/00044"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>The current study investigates the mechanisms involved in nitric oxide (NO)\u2010independent, nonadrenergic, noncholinergic (NANC) inhibitory neurotransmission to the pig urinary bladder neck.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Urothelium\u2010denuded strips were mounted in organ baths containing physiological saline solution (PSS) at 37\u00b0C for isometric force recordings. The relaxations to electrical field stimulation (EFS) were carried out on strips treated with guanethidine, atropine and N<jats:sup>G<\/jats:sup>\u2010nitro\u2010<jats:sc>L<\/jats:sc>\u2010arginine, to block noradrenergic neurotransmission, muscarinic receptors and NO synthase, respectively, and precontracted with phenylephrine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>EFS (1\u201316\u2009Hz) produced frequency\u2010dependent relaxations which were abolished by the blockade of neuronal voltage\u2010activated Na<jats:sup>+<\/jats:sup> channels. Nonselective and selective inhibition of COX and COX\u20101, respectively, and blockade of Na<jats:sup>+<\/jats:sup>\u2013K<jats:sup>+<\/jats:sup> ATPase reduced the EFS\u2010induced relaxations. However, blockade of COX\u20102, soluble guanylyl cyclase, large\u2010, intermediate\u2010 and small\u2010conductance Ca<jats:sup>2+<\/jats:sup>\u2010activated K<jats:sup>+<\/jats:sup> channels, ATP\u2010dependent K<jats:sup>+<\/jats:sup> channels, voltage\u2010gated K<jats:sup>+<\/jats:sup> channels, cAMPc\u2010dependent protein kinase (PKA) and cGMP\u2010dependent protein kinase (PKG) failed to modify the nerve\u2010mediated relaxations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The NO\u2010independent inhibitory neurotransmission to the pig urinary bladder neck is mediated, in part, through prostanoids release from a COX\u20101 pathway, and through activation of the Na<jats:sup>+<\/jats:sup>\u2013K<jats:sup>+<\/jats:sup> ATPase. PKA and PKG pathways and postjunctional K<jats:sup>+<\/jats:sup> channels do not appear to be involved in the NO\u2010independent nerve\u2010mediated relaxations. Neurourol. Urodyn. 30:151\u2013157, 2011. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.20960","type":"journal-article","created":{"date-parts":[[2010,7,23]],"date-time":"2010-07-23T17:43:32Z","timestamp":1279907012000},"page":"151-157","source":"Crossref","is-referenced-by-count":10,"title":["Mechanisms involved in the nitric oxide independent inhibitory neurotransmission to the pig urinary bladder neck"],"prefix":"10.1002","volume":"30","author":[{"given":"Ana","family":"Mart\u00ednez\u2010Sa\u00e9nz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Victoria","family":"Barahona","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis 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