{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:13:53Z","timestamp":1791346433764,"version":"4.3.1"},"reference-count":58,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2011,6,9]],"date-time":"2011-06-09T00:00:00Z","timestamp":1307577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Association between arterial hypertension and urinary bladder dysfunction has been reported in humans and spontaneously hypertensive rats. However, no study exists evaluating the bladder dysfunction in conditions of renovascular hypertension. The purpose of this study was to characterize the bladder dysfunction in two kidney\u2010one clip (2K\u20101C) hypertensive rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A silver clip was placed around the renal artery of male Wistar rats. After 8 weeks, cystometric study, concentration\u2013response curves to contractile and relaxant agents, frequency\u2010dependent contractions, histomorphometry, muscarinic M<jats:sub>2<\/jats:sub>\/M<jats:sub>3<\/jats:sub>mRNA expression and cyclic AMP measurements were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>2K\u20101C rats showed enhanced bladder volume, wall thickness and smooth muscle density. 2K\u20101C rats also exhibited increases in bladder capacity and non\u2010void contractions, and decreases in the inter\u2010contraction intervals. In isolated detrusor smooth muscle (DSM), contractions to carbachol and electrical\u2010field stimulation (EFS) were significantly greater in 2K\u20101C rats. The Rho\u2010kinase inhibitor Y27632 (10\u2009\u00b5M) significantly reduced the carbachol\u2010induced contractions in SHAM and 2K\u20101C rats, but DSM remained overactive in 2K\u20101C rats in presence of Y27632. Concentration\u2010dependent contractions to the P2X receptor agonist \u03b1,\u03b2\u2010methylene ATP, KCl and extracellular Ca<jats:sup>2+<\/jats:sup>did not change between SHAM and 2K\u20101C groups. In 2K\u20101C rats, isoproterenol, metaproterenol and BRL 37\u2010344 (non\u2010selective, \u03b2<jats:sub>2<\/jats:sub>\u2010 and \u03b2<jats:sub>3<\/jats:sub>\u2010selective adrenoceptor agonists, respectively) produced significantly lower relaxations and decreased cAMP levels, whereas relaxant responses to sodium nitroprusside and BAY 41\u20102272 remained unchanged. Muscarinic M<jats:sub>3<\/jats:sub>mRNA expression receptors were higher in 2K\u20101C group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Renovascular hypertensive rats exhibit bladder dysfunction that involves tissue remodeling and enhanced muscarinic M<jats:sub>3<\/jats:sub>\u2010mediated contractions associated with reduced \u03b2\u2010adrenoceptor\u2010mediated signal transduction. Neurourol. Urodynam. 30:1392\u20131402, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.21074","type":"journal-article","created":{"date-parts":[[2011,6,10]],"date-time":"2011-06-10T07:04:40Z","timestamp":1307689480000},"page":"1392-1402","source":"Crossref","is-referenced-by-count":14,"title":["Characterization of the urinary bladder dysfunction in renovascular hypertensive rats"],"prefix":"10.1002","volume":"30","author":[{"given":"Antonio C.S.","family":"Ramos\u2010Filho","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fab\u00edola Z.T.","family":"M\u00f3nica","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla F.","family":"Franco\u2010Penteado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio 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