{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T05:13:14Z","timestamp":1698642794470},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,7,20]],"date-time":"2011-07-20T00:00:00Z","timestamp":1311120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>We investigated expression of tachykinin peptides neurokinin A, neurokinin B, and substance P within urethras of spontaneously hypertensive rats (SHRs) and determined if a traditional Chinese herbal mixture, THC\u2010002, decreased them.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ten\u2010 and 40\u2010week\u2010old male SHRs were randomly separated into three groups (n\u2009=\u200912 each). Rats of one group were given orally 20\u2009ml 0.9% NaCl solution per kg body weight daily for 1 week. One hour later, each received a similar volume of water. Rats in the second group were also given saline. One hour later, each received 20\u2009mg THC\u2010002 per kg body weight. The third group was untreated. The urethras were removed and separated into prostatic and non\u2010prostatic regions and analyzed by real\u2010time reverse transcription polymerase chain reaction (n\u2009=\u20096) and immunohistochemistry (n\u2009=\u20096).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In 40\u2010week\u2010old untreated SHRs, neurokinin B mRNA and protein, and substance P mRNA in prostatic urethras were significantly higher compared to the 10\u2010week\u2010old ones. Neurokinin A mRNA and protein, and substance P protein of the 40\u2010week\u2010old saline\u2010loaded prostatic urethras were significantly higher compared to the 40\u2010week\u2010old untreated ones. In 40\u2010week\u2010old untreated SHRs, the non\u2010prostatic urethral neurokinin B protein was significantly higher compared to the 10\u2010week\u2010old ones. In 40\u2010week\u2010old, saline\u2010loaded SHRs, neurokinin A protein of the non\u2010prostatic urethras was significantly higher compared to 40\u2010week\u2010old the untreated ones. In 40\u2010week\u2010old SHRs, THC\u2010002 significantly decreased the expression of the urethral tachykinins, except for non\u2010prostatic urethral neurokinin A mRNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Aging and saline\u2010loading increased the expression of urethral tachykinin mRNAs and peptides. THC\u2010002 partially decreased them. Neurourol. Urodynam. 31:201\u2013206, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.21179","type":"journal-article","created":{"date-parts":[[2011,7,20]],"date-time":"2011-07-20T13:04:09Z","timestamp":1311167049000},"page":"201-206","source":"Crossref","is-referenced-by-count":2,"title":["A galenical of traditional chinese herbal mixture (THC\u2010002) reduces expression of tachykinin peptides within urethras of spontaneously hypertensive 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