{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:27:01Z","timestamp":1740104821682,"version":"3.37.3"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,9,26]],"date-time":"2011-09-26T00:00:00Z","timestamp":1316995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n, Spain","doi-asserted-by":"crossref","award":["PS09\/00044"],"award-info":[{"award-number":["PS09\/00044"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>There is no information about the signaling pathways involved in the endothelin\u20101 (ET\u20101)\u2010induced contraction of bladder neck. The current study investigates the mechanisms involved in the ET\u20101\u2010elicited contraction in the pig bladder neck.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bladder neck strips were mounted in organ baths containing physiological saline solution at 37\u00b0C and gassed with 95% O<jats:sub>2<\/jats:sub> and 5% CO<jats:sub>2<\/jats:sub>, for isometric force recording to endothelin receptor agonists, noradrenaline (NA), and electrical field stimulation. Endothelin ET<jats:sub>A<\/jats:sub> receptor expression was also determined, by both immunohistochemistry and Western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>ET<jats:sub>A<\/jats:sub> receptor expression (Western blot) was observed in the muscular layer and urothelium. A strong ET<jats:sub>A<\/jats:sub>\u2010immunoreactivity (ET<jats:sub>A<\/jats:sub>\u2010IR) was identified within nerve fibers among smooth muscle bundles. ET\u20101 and ET\u20102 evoked similar concentration\u2010dependent contractions of urothelium\u2010denuded preparations. ET\u20103 produced a slight response, whereas the ET<jats:sub>B<\/jats:sub> receptor agonist BQ3020 failed to promote contraction. BMS182874, an ET<jats:sub>A<\/jats:sub> receptor antagonist, reduced ET\u20101\u2010induced contraction whereas BQ788, an ET<jats:sub>B<\/jats:sub> antagonist, did not change such responses. ET\u20101 contractions were reduced by extracellular Ca<jats:sup>2+<\/jats:sup> removal and by inhibition of voltage\u2010gated Ca<jats:sup>2+<\/jats:sup> (VOC) (L\u2010type) and non\u2010VOC channels, Rho\/Rho\u2010kinase pathway, and neuronal VOC channels. NA produced contractions which were enhanced by ET\u20101 threshold concentrations. ET<jats:sub>A<\/jats:sub> receptor blockade enhanced nitric oxide\u2010dependent nerve\u2010mediated relaxations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results suggest that ET\u20101 produces contraction via muscular ET<jats:sub>A<\/jats:sub> receptors coupled to extracellular Ca<jats:sup>2+<\/jats:sup> entry via VOC (L\u2010type) and non\u2010VOC channels. Intracellular Ca<jats:sup>2+<\/jats:sup> mobilization and a Rho\/Rho\u2010kinase pathway could also be involved in these responses. ET\u20101\u2010evoked potentiation on noradrenergic contraction, and neuronal ET<jats:sub>A<\/jats:sub> receptors modulating nitrergic inhibitory neurotransmission, are also demonstrated. Neurourol. Urodynam. 31:156\u2013161, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.21187","type":"journal-article","created":{"date-parts":[[2011,9,28]],"date-time":"2011-09-28T01:23:12Z","timestamp":1317172992000},"page":"156-161","source":"Crossref","is-referenced-by-count":3,"title":["Mechanisms involved in endothelin\u20101\u2010induced contraction of the pig urinary bladder neck"],"prefix":"10.1002","volume":"31","author":[{"given":"Jos\u00e9 Luis","family":"Arteaga","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis M.","family":"Orensanz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Pilar","family":"Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Victoria","family":"Barahona","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paz","family":"Recio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Mart\u00ednez\u2010S\u00e1enz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00edtor S.","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana S. F.","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albino","family":"Garc\u00eda\u2010Sacrist\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dolores","family":"Prieto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Medardo","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,9,26]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.8.2863"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/348730a0"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.72.3.526"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706604"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(91)91806-N"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20368"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707669"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2010.04.040"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20960"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00314.x"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00144.x"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706832"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20287"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2007.11.021"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002400050134"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)67319-3"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20500"},{"key":"e_1_2_7_19_2","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1042\/cs0960397","article-title":"Pharmacological and molecular biological evidence for ETA endothelin receptor subtype mediating mechanical responses in the detrusor smooth muscle of the human urinary bladder","volume":"96","author":"Okamoto\u2010Koizumi T","year":"1999","journal-title":"Clin Sci (Lond)"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)36895-7"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)38030-8"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00178958"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702640"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20694"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1994.tb14761.x"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(94)00624-G"}],"container-title":["Neurourology and Urodynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.21187","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.21187","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.21187","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T23:59:29Z","timestamp":1697587169000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/nau.21187"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,26]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1002\/nau.21187"],"URL":"https:\/\/doi.org\/10.1002\/nau.21187","archive":["Portico"],"relation":{},"ISSN":["0733-2467","1520-6777"],"issn-type":[{"type":"print","value":"0733-2467"},{"type":"electronic","value":"1520-6777"}],"subject":[],"published":{"date-parts":[[2011,9,26]]}}}