{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:16:39Z","timestamp":1791090999026,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,3,30]],"date-time":"2012-03-30T00:00:00Z","timestamp":1333065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["1P50 DK52620-12"],"award-info":[{"award-number":["1P50 DK52620-12"]}]},{"name":"NIH","award":["R01-DK082719-02"],"award-info":[{"award-number":["R01-DK082719-02"]}]},{"name":"The John W. Duckett Chair"},{"name":"The Leonard and Madlyn Abramson Chair"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>We set out to characterize the voiding phenotypes of male mice to a water avoidance stress (WAS) protocol and compare the molecular changes with those induced by surgically induced partial bladder outlet obstruction (pBOO).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Six\u2010week\u2010old male Swiss Webster mice housed with sibling littermates were individually placed on a platform centered in the middle of a water filled basin for 1\u2009hr daily for 4 weeks. A non stressed cohort of sibling littermates served as controls. Measured end points included voiding frequency, voided volume, bladder mass, and in vivo cystometry. Molecular end points included myosin heavy chain (MHC) isoform distribution by PCR, and nuclear translocation of hypoxia inducible factor (HIF1\u03b1) and the nuclear factor of activated T\u2010cells (NFAT) by gel shift assay. These molecular endpoints were compared with samples from male mice undergoing anatomic pBOO.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>WAS resulted in increased average voided volumes and bladder mass, and a decrease in voiding frequency (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). The slower MHC A isoform was only expressed in the pBOO group that developed severe hypertrophy. Gel shift assays revealed substantial increases in HIF1\u2010\u03b1 nuclear translocation in the group subjected to pBOO that developed severe hypertrophy but minimal changes in the pBOO group that developed minimal hypertrophy and the swim stress groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The WAS model induces moderate bladder wall hypertrophy in the absence of any surgical manipulation. Neurourol. Urodynam. 31:1185\u20131189, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.22207","type":"journal-article","created":{"date-parts":[[2012,4,3]],"date-time":"2012-04-03T14:21:21Z","timestamp":1333462881000},"page":"1185-1189","source":"Crossref","is-referenced-by-count":16,"title":["Water avoidance stress results in an altered voiding phenotype in male mice"],"prefix":"10.1002","volume":"31","author":[{"given":"Erin","family":"McGonagle","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariana","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Butler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Sliwoski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Valentino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas","family":"Canning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen A.","family":"Zderic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,3,30]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.182.4115.939"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.90749.2008"},{"key":"e_1_2_5_4_2","first-page":"11","article-title":"Social stress\u2010induced bladder dysfunction: Potential role of corticotropin\u2010releasing factor","volume":"11","author":"Wood SK","year":"2009","journal-title":"Am J Physiol Regul Integr Comp Physiol"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000138045.61378.96"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(89)90142-3"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-4737-2_11"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00513.2002"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1101-1025"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00274.2002"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00029.2004"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00477.2007"},{"key":"e_1_2_5_13_2","first-page":"20","article-title":"Calcineurin mediates bladder wall remodeling secondary to partial outlet obstruction","volume":"20","author":"Chang AY","year":"2011","journal-title":"Am J Physiol Renal Physiol"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)66307-9"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005392-200101000-00072"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000145891.35810.56"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2007.04.009"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2011.06.041"}],"container-title":["Neurourology and Urodynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.22207","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.22207","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.22207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T02:49:52Z","timestamp":1697597392000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/nau.22207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,30]]},"references-count":17,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2012,9]]}},"alternative-id":["10.1002\/nau.22207"],"URL":"https:\/\/doi.org\/10.1002\/nau.22207","archive":["Portico"],"relation":{},"ISSN":["0733-2467","1520-6777"],"issn-type":[{"value":"0733-2467","type":"print"},{"value":"1520-6777","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,30]]}}}