{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:22:52Z","timestamp":1790432572880,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,9,21]],"date-time":"2012-09-21T00:00:00Z","timestamp":1348185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>We determined if transient receptor potential melastatin 8 (TRPM8) channels are involved in the detrusor overactivity induced by menthol, or exposure to low temperature (LT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two days prior to cystometric investigation, the bladders of 10\u2010week\u2010old Sprague\u2013Dawley rats were cannulated to measure bladder pressure. After a 20\u2009min baseline cystometry period, the TRPM8 channel antagonist, <jats:italic>N<\/jats:italic>\u2010(4\u2010tert\u2010butylphenyl)\u20104\u2010(3\u2010chloropyridin\u20102\u2010yl)piperazine\u20101\u2010carboxamide (BCTC), or vehicle, was administered through a jugular vein catheter (n\u2009=\u20096). A 90% menthol solution was sprayed onto bare leg skin once every 5\u2009min for 20\u2009min, and then cystometric measurements were repeated. After a 30\u2010min recovery period, the rats were intravenously administered 0.1\u2009\u00b5mol\/kg BCTC. Five minutes later, they were again sprayed and cystometry recorded. In separate experiments, cannulated rats were intravenously administered 0.001, 0.01, or 0.1\u2009\u00b5mol\/kg BCTC (n\u2009=\u20096 each dose). Five minutes later, they were exposed to LT (4\u2009\u00b1\u20092\u00b0C) for 20\u2009min of cystometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Menthol spray decreased voiding interval, micturition volume, and bladder capacity in the BCTC\u2010free rats. However after BCTC administration, these effects were prevented. Exposure to LT elicited detrusor overactivity that caused decreased voiding interval, micturition volume, and bladder capacity. However, at 0.01 and 0.1\u2009\u00b5mol\/kg, BCTC inhibited this cold stress\u2010induced detrusor overactivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Since the TRPM8 channel agonist, BCTC, inhibited detrusor overactivity in rats sprayed with the TRPM8 channel agonist, menthol, and the drug also inhibited cold stress\u2010induced detrusor overactivity, we conclude that TRPM8 channels mediate, at least partially, detrusor overactivity elicited by exposure to LT. Neurourol. Urodynam. 32: 500\u2013504, 2013. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.22325","type":"journal-article","created":{"date-parts":[[2012,9,21]],"date-time":"2012-09-21T12:07:15Z","timestamp":1348229235000},"page":"500-504","source":"Crossref","is-referenced-by-count":33,"title":["Functional roles of transient receptor potential melastatin 8 (TRPM8) channels in the cold stress\u2010induced detrusor overactivity pathways in conscious 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