{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:36:00Z","timestamp":1776818160096,"version":"3.51.2"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,12,31]],"date-time":"2012-12-31T00:00:00Z","timestamp":1356912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>We determined if THC\u2010002, a galenical produced from Ba\u2010Wei\u2010Die\u2010Huang\u2010Wan, could increase skin temperature and inhibit detrusor overactivity induced by sudden whole body cooling. Further, we determined if THC\u2010002 could decrease expression of transient receptor potential melastatin 8 (TRPM8) channels associated with the cold responses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Hind leg skin temperature of female 10\u2010week\u2010old Sprague\u2013Dawley rats was measured by thermal imaging. Experimental rats (n\u2009=\u200912) were given oral 100\u2009mg\/kg THC\u2010002 daily for one week, and controls (n\u2009=\u200912) were similarly treated with THC\u2010002\u2010free solution. Afterwards, thermal imaging and cystometric investigations of the freely moving conscious rats were performed at room temperature (RT, 27\u2009\u00b1\u20092\u00b0C) for 20\u2009min. The rats were then transferred to a low temperature (LT, 4\u2009\u00b1\u20092\u00b0C) environment during which thermal imaging and cystometric measurements were taken at 5, 10, 20, 30, and 40\u2009min. Afterward, the skin tissues were harvested to estimate expression levels of TRPM8 channels by immunohistochemistry and real\u2010time reverse\u2010transcription polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The RT skin temperature of THC\u2010002\u2010treated rats was significantly higher than controls. During the first 20\u2009min under LT, the control rats exhibited cold stress\u2010induced detrusor overactivity such as decreased voiding interval and bladder capacity. THC\u2010002 partially inhibited the detrusor overactivity patterns. During the second 20\u2009min, skin temperature was relatively stable, and the detrusor overactivity of both groups slowly disappeared. THC\u2010002 significantly reduced expression of TRPM8 channel protein and mRNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>THC\u2010002 inhibited cold stress\u2010induced detrusor overactivity resulting from decreasing skin temperature. Therefore, THC\u2010002 might provide resistance to cold stress\u2010exacerbated lower urinary tract symptoms. Neurourol. Urodynam. 32: 486\u2013492, 2013. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.22335","type":"journal-article","created":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T20:15:26Z","timestamp":1357071326000},"page":"486-492","source":"Crossref","is-referenced-by-count":13,"title":["A galenical produced from Ba\u2010Wei\u2010Die\u2010Huang\u2010Wan (THC\u2010002) provides resistance to the cold stress\u2010induced detrusor overactivity in conscious rats"],"prefix":"10.1002","volume":"32","author":[{"given":"Tetsuya","family":"Imamura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Ishizuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gautam 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