{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T04:54:37Z","timestamp":1696481677071},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,6,14]],"date-time":"2013-06-14T00:00:00Z","timestamp":1371168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Chi Mei Foundation Hospital, Tainan, Taiwan","award":["CMNCKU9806"],"award-info":[{"award-number":["CMNCKU9806"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>The use of surgical mesh for female pelvic floor reconstruction has increased in recent years. However, there is paucity of information about the biological responses of host stroma cells to different meshes. This study was aimed to establish an in vitro experimental model to study the micro\u2010environment of extracellular matrix (ECM) with embedded mesh and the stroma cell behaviors to different synthetic meshes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Matrigel multi\u2010cellular co\u2010culture system with embedded mesh was used to evaluate the interaction of stroma cells and synthetic mesh in a simulated ECM environment. Human umbilical vein endothelial cells (HUVEC) and NIH3T3 fibroblasts were inoculated in the system. The established multi\u2010cellular Matrigel co\u2010culture system was used to detect stroma cell recruitment and tube formation ability for different synthetic meshes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HUVEC and NIH3T3 cells were recruited into the mesh interstices and organized into tube\u2010like structures in type I mesh material from Perigee, Marlex and Prolift 24\u2009hr after cell inoculation. On the contrary, there was little recruitment of HUVEC and NIH3T3 cells into the type III mesh of intra\u2010vaginal sling (IVS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The Matrigel multi\u2010cellular co\u2010culture system with embedded mesh offers a useful in vitro model to study the biological behaviors of stroma cells in response to different types of synthetic meshes. The system can help to select ideal mesh candidates before actual implantation into the human body. <jats:italic>Neurourol. Urodynam. 33:449\u2013454, 2014<\/jats:italic>. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.22425","type":"journal-article","created":{"date-parts":[[2013,6,14]],"date-time":"2013-06-14T12:52:16Z","timestamp":1371214336000},"page":"449-454","source":"Crossref","is-referenced-by-count":2,"title":["In vitro extracellular matrix model to evaluate stroma cell response to transvaginal mesh"],"prefix":"10.1002","volume":"33","author":[{"given":"Ming\u2010Ping","family":"Wu","sequence":"first","affiliation":[{"name":"Division of Urogynecology and Pelvic Floor Reconstruction, Department of Obstetrics and Gynecology Chi Mei Foundation Hospital Tainan Taiwan"},{"name":"Center of General Education, Chia Nan University of Pharmacy and Science Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuan\u2010Hui","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Chang Gung Memorial Hospital Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng\u2010Yu","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Kaohsiung Municipal Hsiao\u2010Kang Hospital Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chau\u2010Chen","family":"Yang","sequence":"additional","affiliation":[{"name":"Center of General Education, Chia Nan University of Pharmacy and Science Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yat\u2010Ching","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Urology, College of Medicine and Hospital National Cheng Kung University Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,6,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2002.123819"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.4065"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0029-7844(97)00058-6"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2004.06.109"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2002.121897"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/SPV.0b013e3182254cf1"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-006-0090-1"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-008-0697-5"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001703-200210000-00015"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1028-4559(08)60072-8"},{"key":"e_1_2_6_12_1","first-page":"e1","article-title":"Trends in use of surgical mesh for pelvic organ prolapse","volume":"208","author":"Funk MJ","year":"2013","journal-title":"Am J Obstet Gynecol"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2006.03.020"},{"key":"e_1_2_6_14_1","first-page":"577","article-title":"Conceptual advance in the surgical management of gential prolapse: The TVM technique emergence","volume":"33","author":"Berrocal J","year":"2004","journal-title":"J Gynecol Obstet Biol Reprod (Paris)"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1009521"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64203-4"},{"key":"e_1_2_6_17_1","first-page":"6077","article-title":"Volume\u2010sensitive chloride channels associated with human cervical carcinogenesis","volume":"55","author":"Chou CY","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M308232200"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgn077"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-003-0664-2"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21199"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.04.023"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00423-011-0780-0"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0736-5748(92)90007-M"},{"key":"e_1_2_6_25_1","first-page":"1122","article-title":"Alpha\u2010smooth muscle actin expression in cultured lens epithelial cells","volume":"41","author":"Nagamoto T","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm809"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.070112"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06-6755com"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02426382"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11934-005-0057-7"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016340903295576"}],"container-title":["Neurourology and Urodynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.22425","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.22425","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.22425","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T02:58:56Z","timestamp":1696388336000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/nau.22425"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6,14]]},"references-count":30,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1002\/nau.22425"],"URL":"https:\/\/doi.org\/10.1002\/nau.22425","archive":["Portico"],"relation":{},"ISSN":["0733-2467","1520-6777"],"issn-type":[{"value":"0733-2467","type":"print"},{"value":"1520-6777","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,14]]}}}