{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T06:42:43Z","timestamp":1773297763158,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,8,29]],"date-time":"2013-08-29T00:00:00Z","timestamp":1377734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To study micturition and bladder overactivity in female rats after chronic treatment with the fatty acid amide hydrolase (FAAH) inhibitor oleoyl ethyl amide (OEtA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sprague\u2013Dawley rats received daily subcutaneous injections of OEtA (0.3\u2009mg\/kg), or vehicle for 2 weeks. Cystometries, organ bath studies, Western blot, and immunofluorescence were then used. Expressions of FAAH, cannabinoid 1 and 2 receptors (CB1 and CB2), mitogen\u2010activated protein kinase (MAPK), vesicular acetyl choline\u2010transporter protein (VAChT), and calcitonin gene\u2010related peptide (CGRP) were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At baseline, OEtA\u2010treated rats had higher values (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) of micturition intervals (MI) and volumes (MV), bladder capacity (BC), threshold pressure, and flow pressure than vehicle controls. Intravesical PGE<jats:sub>2<\/jats:sub> reduced MI, MV, and BC, and increased basal pressure and the area under the curve in all rats. However, these urodynamic parameters were altered less by intravesical PGE<jats:sub>2<\/jats:sub> in OEtA\u2010treated rats (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05 vs. vehicle controls). Compared to vehicle controls, detrusor from OEtA\u2010treated rats had larger contractions to carbachol at 10\u20130.1\u2009\u00b5M, but no difference in <jats:italic>E<\/jats:italic><jats:sub>max<\/jats:sub> was recorded. FAAH, CB1, CB2, VAChT, or CGRP was similarly expressed in bladders from all rats. In separate experiments, intravesical OEtA increased mucosal expression of phosphorylated MAPK.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Chronic FAAH inhibition altered sensory urodynamic parameters and reduced bladder overactivity. Even if it cannot be excluded that OEtA may act on central nervous sensory pathways to contribute to these effects, the presence of FAAH and CB receptors in the bladder and activation of intracellular signals for CB receptors by intravesical OEtA suggest a local role for FAAH in micturition control. <jats:italic>Neurourol. Urodynam. 33:1251\u20131258, 2014<\/jats:italic>. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.22482","type":"journal-article","created":{"date-parts":[[2013,8,29]],"date-time":"2013-08-29T07:52:58Z","timestamp":1377762778000},"page":"1251-1258","source":"Crossref","is-referenced-by-count":15,"title":["The fatty acid amide hydrolase inhibitor oleoyl ethyl amide counteracts bladder overactivity in female rats"],"prefix":"10.1002","volume":"33","author":[{"given":"Giorgio","family":"Gandaglia","sequence":"first","affiliation":[{"name":"Urological Research Institute San Raffaele Scientific Institute Milan Italy"},{"name":"Department of Clinical and Experimental Pharmacology Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Strittmatter","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Pharmacology Lund University Lund Sweden"},{"name":"Department of Urology 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