{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T14:42:29Z","timestamp":1784644949897,"version":"3.55.0"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,12,29]],"date-time":"2015-12-29T00:00:00Z","timestamp":1451347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NS035790"],"award-info":[{"award-number":["NS035790"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Previous studies have shown that the activin\u2010binding protein follistatin reduces inflammation in several mouse models of colitis. To determine whether follistatin also has a beneficial effect following bladder inflammation, we induced cystitis in mice using cyclophosphamide (CYP) and examined the relationship between bladder hypersensitivity and bladder follistatin expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Adult female C57BL\/6 mice were treated with CYP (100\u2009mg\/kg) or vehicle (saline) three times over 5 days. Bladder hypersensitivity was assessed by recording the visceromotor response (VMR) to urinary bladder distension and in vitro single\u2010fiber bladder afferent recording. Follistatin gene expression was measured using qRT\u2010PCR. Immunohistochemistry was employed for further characterization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Bladder hypersensitivity was established by day 6 and persisted to day 14 in CYP\u2010treated mice. On day 14, hypersensitivity was accompanied by increases in follistatin gene expression in the bladder. Follistatin\u2010like immunoreactivity colocalized with laminin, and the percentage of structures in the lamina propria that were follistatin\u2010positive was increased in CYP\u2010treated mice. Exogenous follistatin increased VMR and afferent responses to bladder distension in CYP\u2010 but not vehicle\u2010treated mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Chronic bladder pain following CYP treatment is associated with increased follistatin expression in the bladder. These results suggest a novel, pro\u2010nociceptive role for follistatin in cystitis, in contrast with its proposed therapeutic role in colitis. This protein has exciting potential as a biomarker and therapeutic target for bladder hypersensitivity. <jats:italic>Neurourol. Urodynam. 36:286\u2013292, 2017<\/jats:italic>. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.22941","type":"journal-article","created":{"date-parts":[[2015,12,29]],"date-time":"2015-12-29T22:04:44Z","timestamp":1451426684000},"page":"286-292","source":"Crossref","is-referenced-by-count":6,"title":["A novel role for follistatin in hypersensitivity following cystitis"],"prefix":"10.1002","volume":"36","author":[{"given":"Amber D.","family":"Shaffer","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Center for Pain Research University of Pittsburgh Pittsburgh Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Center for Pain Research University of Pittsburgh Pittsburgh Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun\u2010Ho","family":"La","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Center for Pain Research University of Pittsburgh Pittsburgh Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonali C.","family":"Joyce","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Center for Pain Research University of Pittsburgh Pittsburgh Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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