{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T09:33:05Z","timestamp":1744277585639},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>This study determined if combined treatment with the muscarinic receptor (MR) antagonist solifenacin and the \u03b2<jats:sub>3<\/jats:sub>\u2010adrenergic receptor (AR) agonist mirabegron could inhibit detrusor overactivity induced by cold stress in spontaneously hypertensive rats (SHRs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010two female 10\u2010week\u2010old SHRs were fed an 8% NaCl\u2010supplemented diet for 4 weeks. Cystometric measurements of the unanesthetized, unrestricted rats were performed at room temperature (RT, 27\u2009\u00b1\u20092\u00b0C) for 20\u2009min. The rats were then intravenously administered vehicle, 0.1\u2009mg\/kg solifenacin alone, 0.1\u2009mg\/kg mirabegron alone, or the combination of 0.1\u2009mg\/kg mirabegron and 0.1\u2009mg\/kg solifenacin (n\u2009=\u20098 each group). Five minutes later, the treated rats were exposed to low temperature (LT, 4\u2009\u00b1\u20092\u00b0C) for 40\u2009min. Finally, the rats were returned to RT. After the cystometric investigations, the \u03b2<jats:sub>3<\/jats:sub>\u2010ARs and M<jats:sub>3<\/jats:sub>\u2010MRs expressed within the urinary bladders were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Just after transfer from RT to LT, vehicle\u2010, solifenacin\u2010, and mirabegron\u2010treated SHRs exhibited detrusor overactivity that significantly decreased voiding interval and bladder capacity. However, treatment with the combination of solifenacin and mirabegron partially inhibited the cold stress\u2010induced detrusor overactivity patterns. The decreases of voiding interval and bladder capacity in the combination\u2010treated rats were significantly inhibited compared to other groups. Within the urinary bladders, there were no differences between expression levels of M<jats:sub>3<\/jats:sub>\u2010MR and \u03b2<jats:sub>3<\/jats:sub>\u2010AR mRNA. The tissue distribution of M<jats:sub>3<\/jats:sub>\u2010MRs was similar to that of the \u03b2<jats:sub>3<\/jats:sub>\u2010ARs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study suggested that the combination of solifenacin and mirabegron act synergistically to inhibit the cold stress\u2010induced detrusor overactivity in SHRs. <jats:italic>Neurourol. Urodynam. 36:1026\u20131033, 2017<\/jats:italic>. \u00a9 2016 The Authors. <jats:italic>Neurourology and Urodynamics<\/jats:italic> Published by Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23061","type":"journal-article","created":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T16:58:59Z","timestamp":1467392339000},"page":"1026-1033","source":"Crossref","is-referenced-by-count":6,"title":["Combined treatment with a \u03b2<sub>3<\/sub>\u2010adrenergic receptor agonist and a muscarinic receptor antagonist inhibits detrusor overactivity induced by cold stress in spontaneously hypertensive rats"],"prefix":"10.1002","volume":"36","author":[{"given":"Tetsuya","family":"Imamura","sequence":"first","affiliation":[{"name":"Department of Urology Shinshu University School of Medicine Matsumoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teruyuki","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Department of Urology Shinshu 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