{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:23:03Z","timestamp":1790036583748,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,10,29]],"date-time":"2016-10-29T00:00:00Z","timestamp":1477699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"crossref","award":["2013\/13907-4"],"award-info":[{"award-number":["2013\/13907-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To evaluate the effects of the beta\u20103 adrenoceptor agonist, mirabegron in a mouse model of detrusor overactivity induced by obesity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>C57BL\/6 male mice were fed with standard chow or high\u2010fat diet for 12 weeks. Lean and obese mice were treated orally with mirabegron (10\u2009mg\/kg\/day) from the last 2 weeks of diet. Cystometric evaluations, functional assays, protein expression for phosphodiesterase type 4 (PDE4), and cyclic adenosine monophosphate (cAMP) measurement were carried out.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In obese mice the body weight, epididymal fat mass, fasting glucose, and low\u2010density lipoprotein (LDL) levels were higher (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.001) than in the lean mice. A reduction of 34% and 54% and an increase of 35% in the epididimal fat, LDL, and HDL levels (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05), respectively, were observed in the obese group treated with mirabegron, whereas no changes were seen in the lipid profile from lean mice. Obese group showed irregular micturition pattern, characterized by significant increases in frequency and non\u2010void contractions. Carbachol, potassium chloride, and electrical\u2010field stimulation induced detrusor smooth muscle (DSM) contractions, which were greater in bladders from obese mice than from lean mice. Two\u2010week treatment with mirabegron restored all the contractile response alterations in the DSM. Basal intracellular levels of cAMP were reduced (68%), whereas PDE4 protein expression was increased (54%) in bladder from obese mice. Mirabegron restored the cAMP levels in obese bladder, without changing the PDE4 expression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Mirabegron was able to completely restore the urinary alterations seen in the bladder from obese mice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/nau.23171","type":"journal-article","created":{"date-parts":[[2016,10,29]],"date-time":"2016-10-29T05:23:01Z","timestamp":1477718581000},"page":"1511-1518","source":"Crossref","is-referenced-by-count":24,"title":["Long\u2010term treatment with the beta\u20103 adrenoceptor agonist, mirabegron ameliorates detrusor overactivity and restores cyclic adenosine monophosphate (cAMP) levels in obese mice"],"prefix":"10.1002","volume":"36","author":[{"given":"Fabiano B.","family":"Calmasini","sequence":"first","affiliation":[{"name":"Department of Pharmacology Faculty of Medical Sciences University of Campinas (UNICAMP) Campinas SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana G.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Medical Sciences University of Campinas (UNICAMP) Campinas SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo C.","family":"Alexandre","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Medical Sciences University of Campinas (UNICAMP) Campinas SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio H.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Medical Sciences University of Campinas (UNICAMP) Campinas SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmem P.V.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Medical Sciences University of Campinas (UNICAMP) Campinas SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuany Z.","family":"Candido","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Medical Sciences University of Campinas (UNICAMP) Campinas SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edson","family":"Antunes","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Medical Sciences University of Campinas (UNICAMP) Campinas SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fab\u00edola Z.","family":"M\u00f3nica","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Medical Sciences University of Campinas (UNICAMP) Campinas SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2014.732"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.288.14.1758"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2009.09.083"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.10052"},{"key":"e_1_2_8_6_1","first-page":"1","article-title":"Current and future pharmacotherapy for treating overactive bladder","volume":"2","author":"Thiagamoorthy G","year":"2016","journal-title":"Expert Opin Pharmacother"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.106.115840"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.22930"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-013-0900-1"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2012.11240.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2013.06.043"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-015-1139-9"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2012.11.060"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048507"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000111245.75752.C6"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2007.05.114"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2013.251843"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-012-0821-4"},{"key":"e_1_2_8_19_1","unstructured":"Department of Health and Human Services U.S. Food and Drug Administration.2012. 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