{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:01:22Z","timestamp":1758819682911,"version":"3.37.3"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,3,27]],"date-time":"2017-03-27T00:00:00Z","timestamp":1490572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo-FAPESP","award":["2011\/51868-5"],"award-info":[{"award-number":["2011\/51868-5"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Investigate the effect of a novel cell\u2010based therapy with skeletal muscle\u2010derived mononuclear cells (SMDMCs) in a rat model of stress urinary incontinence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male Wistar\u2010Kyoto rats\u2019 hind limb muscles were enzymatically dissociated, and SMDMCs were isolated without needing expansion. The cell population was characterized. Twenty female rats underwent urethrolysis. One week later, 10 rats received periurethral injection of 10<jats:sup>6<\/jats:sup> cells (SMDMC group), and 10 rats received saline injections (Saline group). Ten rats underwent sham surgery (Sham group). Four weeks after injection, animals were euthanized and the urethra was removed. The incorporation of SMDMCs in the female urethra was evaluated with fluorescence in situ hybridization for the detection of Y\u2010chromosomes. Hematoxylin and eosin, Masson's trichrome staining, and immunohistochemistry for actin and myosin were performed. The muscle\/connective tissue, actin and myosin ratios were calculated. Morphological evaluation of the urethral diameters and fractional areas of the lumen, mucosa, and muscular layer was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SMDMCs population was consistent with the presence of muscle cells, muscle satellite cells, perivascular cells, muscle progenitor cells, and endothelial cells. SMDMCs were incorporated into the urethra. A significant decrease in the muscle\/connective tissue ratio was observed in the Saline group compared with the SMDMC and Sham groups. The proportions of actin and myosin were significantly decreased in the Saline group. No differences were observed in the morphometric parameters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>SDMSC were incorporated into the rat urethra and promoted histological recovery of the damaged urethral sphincter, resulting in decreased connective tissue deposition and increased muscle content.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23262","type":"journal-article","created":{"date-parts":[[2017,3,27]],"date-time":"2017-03-27T15:54:56Z","timestamp":1490630096000},"page":"2011-2018","source":"Crossref","is-referenced-by-count":3,"title":["Periurethral muscle\u2010derived mononuclear cell injection improves urethral sphincter restoration in rats"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5246-1496","authenticated-orcid":false,"given":"Marcelo Pitelli","family":"Turco","sequence":"first","affiliation":[{"name":"Division of Urology University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex Balduino","family":"de Souza","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine University of Sao Paulo Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isida","family":"de Campos Sousa","sequence":"additional","affiliation":[{"name":"Division of Urology University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Fratini","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine University of Sao Paulo Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana Matera","family":"Veras","sequence":"additional","affiliation":[{"name":"Department of Pathology School of Medicine University of S\u00e3o Paulo Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcio Nogueira","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine University of Sao Paulo Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"Jos\u00e9","family":"de Bessa","sequence":"additional","affiliation":[{"name":"Division of Urology University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina Pandolphi","family":"Brolio","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine University of Sao Paulo Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katia Ramos Moreira","family":"Leite","sequence":"additional","affiliation":[{"name":"Division of Urology University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Homero","family":"Bruschini","sequence":"additional","affiliation":[{"name":"Division of Urology University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Srougi","sequence":"additional","affiliation":[{"name":"Division of Urology University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Ang\u00e9lica","family":"Miglino","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine University of Sao Paulo Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiano Mendes","family":"Gomes","sequence":"additional","affiliation":[{"name":"Division of Urology University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,3,27]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"AbramsPCL KhouryS WeinA. Incontinence: 5th International Consultation on Incontinence Paris. France: ICUD\u2010EUA2012."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20798"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcna.2010.08.022"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOU.0b013e3283476eb5"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2010007500112"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/14653240903350265"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.2010.02471.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2010.25.5.663"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2010.25.6.813"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2013-0197"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.09.028"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2011.0621"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07384"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00760.2004"},{"key":"e_1_2_8_16_1","unstructured":"Percoll.http:\/\/www.gelifesciences.com\/gehcls_images\/GELS\/Related%20Content\/Files\/1314735988470\/litdoc18111569_20161014015502.pdf"},{"key":"e_1_2_8_17_1","first-page":"277","volume-title":"Unbiased Stereology: Three\u2010Dimensional Measurement in Microscopy","author":"Howard V","year":"2005"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1988.tb05320.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001703-200410000-00007"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-011110-130230"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-200205000-00022"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e318170572b"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/612471"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3791\/50846"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.4161\/org.7.2.16150"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-015-9590-z"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1334"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12370"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/GCO.0b013e32833e499d"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.2144\/000112811"}],"container-title":["Neurourology and Urodynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnau.23262","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.23262","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T02:56:59Z","timestamp":1696042619000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/nau.23262"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,27]]},"references-count":29,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1002\/nau.23262"],"URL":"https:\/\/doi.org\/10.1002\/nau.23262","archive":["Portico"],"relation":{},"ISSN":["0733-2467","1520-6777"],"issn-type":[{"type":"print","value":"0733-2467"},{"type":"electronic","value":"1520-6777"}],"subject":[],"published":{"date-parts":[[2017,3,27]]}}}