{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T23:03:46Z","timestamp":1770678226977,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,8,7]],"date-time":"2017-08-07T00:00:00Z","timestamp":1502064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate the effect of tadalafil on bladder blood flow and lower urinary tract function in a rat model of diabetes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>We studied female Sprague\u2010Dawley rats and induced diabetes in some using a single intraperitoneal injection of streptozotocin. We divided the rats into nondiabetes (ND), diabetes (D), and diabetes with tadalafil (DT) groups. The rats were raised for an additional 7 weeks after diabetes induction. The DT group received oral tadalafil (2 mg\/kg\/day) for 7 days before the experiments. At 7 weeks after diabetes induction, we performed cystometry, resected the bladders for immunohistochemistry (hypoxia\u2010inducible factor\u20101\u03b1 [HIF\u20101\u03b1] and 8\u2010oxo\u20102\u2032\u2010deoxyguanosine [8\u2010OHdG] staining), and measured bladder blood supply using a laser blood flow meter.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The opening pressure, when the urethra opens and urine flow starts, was significantly lower in the DT group than in the D group (24.9\u2009\u00b1\u20095.9 vs 43.6\u2009\u00b1\u200912.3 cmH<jats:sub>2<\/jats:sub>O). The inter\u2010contraction interval was significantly longer in the D group than in the ND and DT groups (1566.2\u2009\u00b1\u2009168.7 vs 702.9\u2009\u00b1\u2009165.2 and 787.4\u2009\u00b1\u2009148.8 s). Immunohistochemistry showed positive staining of the urothelial layer for both HIF\u20101\u03b1 and 8\u2010OHdG in the D group, but not in the ND or DT groups. Bladder blood flow was significantly lower in the D group than in the ND or DT groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Tadalafil improves bladder blood supply and lower urinary tract function in diabetic rats. Tadalafil may be a promising drug that restores lower urinary tract dysfunction in the early phase of diabetes.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23372","type":"journal-article","created":{"date-parts":[[2017,8,7]],"date-time":"2017-08-07T09:02:44Z","timestamp":1502096564000},"page":"666-672","source":"Crossref","is-referenced-by-count":20,"title":["Tadalafil, a phosphodiesterase type 5 inhibitor, improves bladder blood supply and restores the initial phase of lower urinary tract dysfunction in diabetic rats"],"prefix":"10.1002","volume":"37","author":[{"given":"Daisuke","family":"Gotoh","sequence":"first","affiliation":[{"name":"Department of Urology Nara Medical University Kashihara Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2870-0466","authenticated-orcid":false,"given":"Kazumasa","family":"Torimoto","sequence":"additional","affiliation":[{"name":"Department of Urology Nara Medical University Kashihara Nara 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