{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:26:57Z","timestamp":1740104817386,"version":"3.37.3"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,10,17]],"date-time":"2017-10-17T00:00:00Z","timestamp":1508198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP17K11175"],"award-info":[{"award-number":["JP17K11175"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>We evaluated the association between lower urinary tract symptoms (LUTS) and the expression of connexin (Cx) and transient receptor potential (TRP) channel on urothelial cells non\u2010invasively collected from voided urine in humans.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 55 patients (36 males and 19 females, median age: 71 years old), who were followed up at University of Yamanashi Hospital, were enrolled in the present study. Urothelial cells were collected from voided urine of patients, and the mRNA expression of each subtype of Cxs and TRP channels was measured using quantitive real\u2010time reverse transcription polymerase chain reaction. We then analyzed the correlation between the expression of Cxs and TRP channels and symptom scores in International Prostate Symptom Scoreand Overactive Bladder Symptom Score, in addition to Interstitial Cystitis Symptom Index (ICSI) from only interstitial cystitis (IC) patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Non\u2010adjusted statistical procedure using Spearman's rank\u2010correlation showed that there were significant correlations between the following expressions and symptom scores; (positive correlations) Cx26 versus urgency score, Cx40 versus nocturia, TRPM2 versus intermittency, TRPV1 versus urge incontinence, (negative correlation) Cx40 versus intermittency, TRPM7 versus pollakisuria. However, a multiple comparison adjustment using Bonferroni correction showed that only Cx40 had a trend of correlation with nocturia in ICSI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The expressions of Cxs and TRP channels on urothelial cells in voided urine could be related to LUTS. Further analysis of urothelial cells in voided urine has the potential to reveal the mechanism of the LUTS and develop new markers with non\u2010invasive methods.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23436","type":"journal-article","created":{"date-parts":[[2017,10,17]],"date-time":"2017-10-17T14:17:54Z","timestamp":1508249874000},"page":"1137-1143","source":"Crossref","is-referenced-by-count":2,"title":["Development of novel and non\u2010invasive diagnostic markers for lower urinary tract symptoms using urothelial cells in voided urine"],"prefix":"10.1002","volume":"37","author":[{"given":"Hiroshi","family":"Shimura","sequence":"first","affiliation":[{"name":"Department of Urology University of Yamanashi Graduate School of Medical Science Chuo Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0408-3678","authenticated-orcid":false,"given":"Takahiko","family":"Mitsui","sequence":"additional","affiliation":[{"name":"Department of Urology University of Yamanashi Graduate School of Medical Science Chuo Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachiko","family":"Tsuchiya","sequence":"additional","affiliation":[{"name":"Department of Urology University of Yamanashi Graduate School of Medical Science Chuo Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Department of Urology Fujiyoshida Municipal Medical Center Fujiyoshida Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Ihara","sequence":"additional","affiliation":[{"name":"Department of Urology University of Yamanashi Graduate School of Medical Science Chuo Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5282-3832","authenticated-orcid":false,"given":"Satoru","family":"Kira","sequence":"additional","affiliation":[{"name":"Department of Urology University of Yamanashi Graduate School of Medical Science Chuo Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5584-0451","authenticated-orcid":false,"given":"Hiroshi","family":"Nakagomi","sequence":"additional","affiliation":[{"name":"Department of Urology University of Yamanashi Graduate School of Medical Science Chuo Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norifumi","family":"Sawada","sequence":"additional","affiliation":[{"name":"Department of Urology University of Yamanashi Graduate School of Medical Science Chuo Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Imai","sequence":"additional","affiliation":[{"name":"Department of Urology University of Yamanashi Graduate School of Medical Science Chuo Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takanori","family":"Mochizuki","sequence":"additional","affiliation":[{"name":"Department of Urology University of Yamanashi Graduate School of Medical Science Chuo Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayuki","family":"Takeda","sequence":"additional","affiliation":[{"name":"Department of Urology University of Yamanashi Graduate School of Medical Science Chuo Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,17]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"193","article-title":"Receptors, channels, and signalling in the urothelial sensory system in the bladder. 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