{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T17:03:03Z","timestamp":1771002183651,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,1,15]],"date-time":"2018-01-15T00:00:00Z","timestamp":1515974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Grant-in-Aid for Scientific Research","award":["15K01376"],"award-info":[{"award-number":["15K01376"]}]},{"name":"Grant-in-Aid for Scientific Research","award":["15K01377"],"award-info":[{"award-number":["15K01377"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>We aimed to investigate the age\u2010associated changes in movement coordination between the urinary bladder and the urethra in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 17 female Sprague\u2010Dawley rats were used. The rats were divided into young (3 months old) and middle\u2010aged (12\u201015 months old) groups. In both groups, isovolumetric cystometry and urethral perfusion pressure (UPP) measurements were performed under urethane anesthesia. After the rhythmic bladder contractions stabilized, L\u2010arginine, a nitric oxide (NO) substrate (100 mg\/kg), was administered intravenously in both groups. Subsequently, N\u2010nitro\u2010L\u2010arginine methyl ester hydrochloride (L\u2010NAME) (50 mg\/kg) was injected intravenously to inhibit NO synthase activity in both groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>UPP change, defined as UPP nadir minus baseline UPP, was significantly smaller in middle\u2010aged rats (64%) than in young rats (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). The mean amplitude of high\u2010frequency oscillations (HFOs) of the external urethral sphincter was also significantly lower (62%) in middle\u2010aged rats than that in young rats (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Urethral contraction during UPP change was also noted in middle\u2010aged rats. This urethral contraction disappeared after L\u2010arginine administration. UPP nadir during bladder contraction was inhibited by L\u2010NAME in both groups. UPP change was greater in middle\u2010aged rats than in young rats, and 3 out of 9 middle\u2010aged rats showed a detrusor\u2010sphincter dyssynergia pattern after L\u2010NAME.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results indicated that aging induces dysfunction in movement coordination between the urinary bladder and the urethra. Thus, age\u2010associated urethral dysfunctions may lead to inefficient voiding with increased post\u2010void residual urine volume, which is often observed in elderly populations.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23481","type":"journal-article","created":{"date-parts":[[2018,1,15]],"date-time":"2018-01-15T08:32:39Z","timestamp":1516005159000},"page":"1313-1319","source":"Crossref","is-referenced-by-count":8,"title":["Age\u2010associated urethral dysfunction in urethane\u2010anesthetized rats"],"prefix":"10.1002","volume":"37","author":[{"given":"Ryu","family":"Kimura","sequence":"first","affiliation":[{"name":"Department of Urology Graduate School of Medicine University of the Ryukyus Okinawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3669-4510","authenticated-orcid":false,"given":"Minoru","family":"Miyazato","sequence":"additional","affiliation":[{"name":"Department of Urology Graduate School of Medicine University of the Ryukyus Okinawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asuka","family":"Ashikari","sequence":"additional","affiliation":[{"name":"Department of Urology Graduate School of Medicine University of the Ryukyus Okinawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuma","family":"Oshiro","sequence":"additional","affiliation":[{"name":"Department of Urology Graduate School of Medicine University of the Ryukyus Okinawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiichi","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Urology Graduate School of Medicine University of the Ryukyus Okinawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1002\/j.2040-4603.2015.tb00596.x","article-title":"Neural control of the lower urinary tract","volume":"5","author":"de Groat WC","year":"2015","journal-title":"Compr Physiol"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-63247-0.00005-5"},{"key":"e_1_2_7_4_1","first-page":"S3","article-title":"The aging bladder","volume":"1","author":"Siroky MB","year":"2004","journal-title":"Rev Urol"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.4111\/kju.2012.53.5.342"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2006.11.019"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2014.08.009"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.1997.tb00156.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.41.1.691"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000121283.92963.05"},{"key":"e_1_2_7_11_1","first-page":"307","article-title":"[Pharmacological analysis of neurotransmitters contributing to lower urinary tract function]. 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