{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T16:56:56Z","timestamp":1780678616555,"version":"3.54.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T00:00:00Z","timestamp":1516752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006510","name":"Duke University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006510","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006510","name":"Duke University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006510","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["K12\u2010DK100024"],"award-info":[{"award-number":["K12\u2010DK100024"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>To assess the feasibility of using voided urine samples to perform a DNA methylation study in females with interstitial cystitis\/bladder pain syndrome (IC\/BPS) as compared to age\u2010 and race\u2010matched controls. A unique methylation profile could lead to a non\u2010invasive, reproducible, and objective biomarker that would aid clinicians in the diagnosis of IC\/BPS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Nineteen IC\/BPS patients and 17 controls were included. IC\/BPS patients had an Interstitial Cystitis Symptom Index score of &gt;8; controls had no bladder symptoms. DNA was extracted from pelleted urine sediment. Samples with &gt;500\u2009ng of genomic DNA underwent quantitative DNA methylation assessment using the Illumina Infinium MethylationEPIC BeadChip. Age\u2010 and race\u2010matching was applied prior to analysis. Linear regression models were used to compare average methylation between IC\/BPS cases and controls at each cytosine guanine dinucleotide site (loci where methylation can occur).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sixteen participants (eight IC\/BPS age\u2010 and race\u2010matched to eight controls) had adequate DNA for methylation analysis. The median age was 43.5 years (interquartile range 33.8, 65.0), the median BMI was 27.1 (IQR 22.7, 31.4), and 14 were Caucasian (87.5%). A total of 688\u2009417 CpG sites were analyzed. In exploratory pathway analysis utilizing the top 1000 differentially methylated CpG sites, the mitogen\u2010activated protein kinase (MAPK) pathway was overrepresented by member genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results demonstrate the feasibility of using voided urine specimens from women with IC\/BPS to perform DNA methylation assessments. Additionally, the data suggest genes within or downstream of the MAPK pathway exhibit altered methylation in IC\/BPS.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23489","type":"journal-article","created":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T10:43:17Z","timestamp":1516790597000},"page":"1485-1493","source":"Crossref","is-referenced-by-count":15,"title":["A genome\u2010scale DNA methylation study in women with interstitial cystitis\/bladder pain syndrome"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9935-5132","authenticated-orcid":false,"given":"Megan S.","family":"Bradley","sequence":"first","affiliation":[{"name":"Division of Urogynecology Department of Obstetrics, Gynecology, and Reproductive Sciences Magee Womens Hospital\u2014University of Pittsburgh School of Medicine Pittsburgh Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily 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