{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T00:42:54Z","timestamp":1776472974174,"version":"3.51.2"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,7,27]],"date-time":"2018-07-27T00:00:00Z","timestamp":1532649600000},"content-version":"vor","delay-in-days":56,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>To evaluate the effects of a treatment with leuprolide acetate (LA) on bladder overactivity as well as the expression of gonadotropin releasing hormone receptor (GnRH\u2010R), and neurofilaments NF68 and NF200 in female rats with overactive bladder induced by castration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Changes in the urodynamic parameters were determined in SHAM, ovariectomized (OVX) and ovariectomized rats treated with LA (OVX\u2010LA). A semi\u2010quantitative analysis for the expression pattern of GnRH\u2010R and neurofilaments NF68 and NF200 were determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Forty\u2010three days after ovariectomy, rats from the OVX group have significant lower values for intercontractile interval (ICI) and compliance (C); as well as higher values for basal bladder pressure (BP) and frequency of non\u2010voiding contractions (NVC). The systemic application of LA increased voiding volume (Vv) and pressure threshold (ThP) in the OVX\u2010LA animals. The application of LA reduced the high frequency of NVC in the OVX rats. No significant differences were found for Vv and NVCs between the OVX\u2010LA vs SHAM groups. At the mid part of the bladder, the presence of GnRH\u2010R was evidenced in the urothelium of the SHAM group. The OVX animals showed different pattern of immunolabeling for GnRH\u2010R as well as for neurofilaments NF200 and NF68, whereas in the OVX\u2010LA group the immunofluorescence pattern was similar to the one seen in SHAM bladders (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05 for OVX vs OVX\u2009+\u2009LA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>the results suggest that systemic application of LA can improve bladder dysfunction in castrated rats, and perhaps considered as a treatment for overactive bladder conditions secondary to menopause.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23505","type":"journal-article","created":{"date-parts":[[2018,7,27]],"date-time":"2018-07-27T16:01:57Z","timestamp":1532707317000},"page":"1574-1582","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Administration of leuprolide acetate, a GnRH agonist, improves urodynamic parameters in ovariectomized rats"],"prefix":"10.1002","volume":"37","author":[{"given":"Daniel","family":"Medina\u2010Agui\u00f1aga","sequence":"first","affiliation":[{"name":"Laboratory of Neurophysiology, Department of Physiology and Pharmacology Autonomous University of Aguascalientes Aguascalientes M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alvaro","family":"Munoz","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Program\u2010Urology Houston Methodist Research Institute Houston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maricela","family":"Luna","sequence":"additional","affiliation":[{"name":"Laboratory of Hormones, Department Cellular and Molecular Neurobiology, Institute of Neurobiology, Juriquilla Campus National Autonomous University of M\u00e9xico Quer\u00e9taro M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos G.","family":"Martinez\u2010Moreno","sequence":"additional","affiliation":[{"name":"Laboratory of Hormones, Department Cellular and Molecular Neurobiology, Institute of Neurobiology, Juriquilla Campus National Autonomous University of M\u00e9xico Quer\u00e9taro M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9s","family":"Quintanar\u2010Stephano","sequence":"additional","affiliation":[{"name":"Laboratory of Neuroimmunoendocrinology, Department of Physiology and Pharmacology Autonomous University of Aguascalientes Aguascalientes M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1257-7723","authenticated-orcid":false,"given":"J. 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