{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:40:37Z","timestamp":1790732437349,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,3,8]],"date-time":"2019-03-08T00:00:00Z","timestamp":1552003200000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2018,3,8]],"date-time":"2018-03-08T00:00:00Z","timestamp":1520467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["W81XWH\u201017\u20101\u20100403"],"award-info":[{"award-number":["W81XWH\u201017\u20101\u20100403"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P01DK093424"],"award-info":[{"award-number":["P01DK093424"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate the role of nerve growth factor (NGF) in lower urinary tract dysfunction in mice with spinal cord injury (SCI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using 4\u2010week SCI mice, single\u2010filling cystometry and external urethral sphincter (EUS)\u2010electromyography were performed under an awake condition. In some SCI mice, anti\u2010NGF antibodies (10\u2009\u00b5g\/kg\/h) were administered for 1 or 2 weeks before the urodynamic study. NGF levels in the bladder and L6\/S1 spinal cord were assayed by ELISA. The transcript levels of P2X receptors and TRP channels in L6\/S1 dorsal root ganglia (DRG) were measured by RT\u2010PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In SCI mice, the area under the curve of non\u2010voiding contractions (NVCs) during the storage phase was significantly decreased in both 1\u2010 and 2\u2010week anti\u2010NGF antibody\u2010treated SCI groups. However, EUS\u2010electromyogram parameters during voiding were not altered by the treatment. Bladder mucosal and spinal NGF levels were decreased after 2 weeks of anti\u2010NGF antibody treatment. TRPA1 and TRPV1 transcripts in L6\/S1 DRG were significantly decreased after 1\u2010 or 2\u2010week anti\u2010NGF treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In SCI mice, NGF is involved in the emergence of NVCs in association with increased expression of TRP receptors that are predominantly found in C\u2010fiber afferent pathways. Thus, NGF targeting treatments could be effective for treating storage problems such as detrusor overactivity after SCI.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23539","type":"journal-article","created":{"date-parts":[[2018,3,8]],"date-time":"2018-03-08T09:36:47Z","timestamp":1520501807000},"page":"1889-1896","source":"Crossref","is-referenced-by-count":36,"title":["The effect of neutralization of nerve growth factor (NGF) on bladder and urethral dysfunction in mice with spinal cord injury"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9587-6798","authenticated-orcid":false,"given":"Naoki","family":"Wada","sequence":"first","affiliation":[{"name":"Department of Urology University of Pittsburgh School of Medicine Pittsburgh Pennsylvania"},{"name":"Department of Renal and Urologic Surgery Asahikawa Medical University Asahikawa 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