{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T02:47:30Z","timestamp":1778035650344,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,8,28]],"date-time":"2018-08-28T00:00:00Z","timestamp":1535414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>To identify the urethral migration of muscle derived stem cells (MDSCs) after intravenous (IV) injection in rats that underwent vaginal distension (VD) and to analyze the effects of MDSC in the urethra of rats after trauma in regards to: (1) mRNA expression of collagens, <jats:italic>Vegf<\/jats:italic>, <jats:italic>Ngf<\/jats:italic>, <jats:italic>Ki67<\/jats:italic>, <jats:italic>Myh11<\/jats:italic>, and <jats:italic>Myh2<\/jats:italic>; (2) expression of smooth and striated muscle proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>MDSCs expressing green fluorescent protein (GFP) were injected into the tail vein of rats 3 days after VD. The location of GFP cells was verified at 2\u2009h and at 7 days following IV injection. Urethras of three groups were analyzed: Control, Trauma 7D, and MDSC 7D. Real\u2010time RT\u2010qPCR and immunohistochemistry were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MDSCs were identified only after 2\u2009h of the procedure in the urethra. <jats:italic>Myh11<\/jats:italic> gene was overexpressed in the Trauma group in relation to Control. <jats:italic>Ki67<\/jats:italic> gene expression was increased in the MDSC group relative to Trauma and Control. <jats:italic>Col1a1<\/jats:italic> and <jats:italic>Col3a1<\/jats:italic> genes expression were increased in the MDSC group relative to Control. <jats:italic>Ngf<\/jats:italic> mRNA level was decreased in the MDSC group in relation to Trauma. Protein expression of Mhy11, Myh2, and Desmin were increased in the MDSC group in relation to Trauma and decreased in the Trauma in relation to Control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MDSCs migrated early to the traumatized urethra, but did not integrate into the tissue. MDSC alters the expression of genes related to cell proliferation, neural growth factor and extracellular matrix and the expression of smooth and striated muscle proteins in the traumatized rat urethra.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23567","type":"journal-article","created":{"date-parts":[[2018,8,30]],"date-time":"2018-08-30T02:16:16Z","timestamp":1535595376000},"page":"2151-2159","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Molecular and immunohistochemical analysis of the urethra of female rats after induced trauma and intravenous therapy with muscle derived stem cells"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1014-1555","authenticated-orcid":false,"given":"Andreisa P.M.","family":"Bilhar","sequence":"first","affiliation":[{"name":"Department of Gynecology Federal University of S\u00e3o Paulo S\u00e3o Paulo 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