{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T10:06:37Z","timestamp":1777457197755,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T00:00:00Z","timestamp":1523923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BPD\/108468\/2015"],"award-info":[{"award-number":["SFRH\/BPD\/108468\/2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To study the contribution of urethral serotonin for the urethro\u2010vesical crosstalk<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Urethane\u2010anesthetized female rats and TPH1\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice underwent isovolumetric or urethral\u2010opened cystometries during intravesical or intraurethral infusion of saline or serotonin solutions. Human and rat bladders and urethrae were immunoreacted against serotonin and neuronal markers. Serotonin concentration and TPH1 mRNA were determined in rat tissue by HPLC and qPCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In rats, under isovolumetric conditions, intraurethral serotonin infusion, but not saline, evoked bladder contractions. This was abolished by urethral anesthesia and by treatment with serotonin receptor antagonists. Serotonin infusion into the bladder had no effect. Under urethral\u2010opened conditions, serotonin infusion reduced the frequency and increased the amplitude of reflex voiding contractions, compared to saline infusion. TPH1\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice, under urethral\u2010opened conditions, exhibited increased frequency and reduced amplitude of voiding contractions compared to WT. Serotonin concentration and TPH1 mRNA expression were higher in the urethra than in the bladder. Cells 5\u2010HT\n                      <jats:sup>+<\/jats:sup>\n                      were found in the human and rat urethral epithelium, close to a sub\u2010epithelial network of cholinergic and sensory fibers, but not in the bladder.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Serotonin, produced and released by urethral cells activates an urethro\u2010vesical pathway that enhances bladder reflex contractions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/nau.23590","type":"journal-article","created":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T07:39:17Z","timestamp":1523950757000},"page":"2389-2397","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Evidence for an urethro\u2010vesical crosstalk mediated by serotonin"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3286-0262","authenticated-orcid":false,"given":"Ana","family":"Coelho","sequence":"first","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Translational Neuro\u2010urology Group, Universidade do Porto Porto Portugal"},{"name":"Instituto de Biologia Molecular e Celular Universidade do Porto Porto Portugal"},{"name":"Faculdade de Medicina, Departmento de Biomedicina Unidade de Biologia Experimental, Universidade do Porto Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Translational Neuro\u2010urology Group, Universidade do Porto Porto Portugal"},{"name":"Instituto de Biologia Molecular e Celular Universidade do Porto Porto Portugal"},{"name":"Faculdade de Medicina, Departmento de Biomedicina Unidade de Biologia Experimental, Universidade do Porto Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helena","family":"Cavaleiro","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, Departmento de Biomedicina Unidade de Biologia Experimental, Universidade do Porto Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celia Duarte","family":"Cruz","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Translational Neuro\u2010urology Group, Universidade do Porto Porto Portugal"},{"name":"Instituto de Biologia Molecular e Celular Universidade do Porto Porto Portugal"},{"name":"Faculdade de Medicina, Departmento de Biomedicina Unidade de Biologia Experimental, Universidade do Porto Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Cruz","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Translational Neuro\u2010urology Group, Universidade do Porto Porto Portugal"},{"name":"Instituto de Biologia Molecular e Celular Universidade do Porto Porto Portugal"},{"name":"Hospital de S. 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