{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T12:04:55Z","timestamp":1779365095856,"version":"3.53.0"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,8,10]],"date-time":"2018-08-10T00:00:00Z","timestamp":1533859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15K15583"],"award-info":[{"award-number":["15K15583"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>To clarify the roles of hydrogen sulfide (H<jats:sub>2<\/jats:sub>S), an endogenous gasotransmitter, in the rat bladder and prostate, we investigated the distribution of enzymes related to H<jats:sub>2<\/jats:sub>S biosynthesis (cystathionine \u03b2\u2010synthase [CBS], cystathionine \u03b3\u2010lyase [CSE], 3\u2010mercaptopyruvate sulfurtransferase [MPST], cysteine aminotransferase [CAT], and D\u2010amino acid oxidase [DAO]) and the content of H<jats:sub>2<\/jats:sub>S. We also investigated the effects of H<jats:sub>2<\/jats:sub>S donors (NaHS and GYY4137) on the contractility of both tissues and on micturition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The distribution of these enzymes was investigated by real\u2010time PCR, Western blot, and immunohistochemistry. Tissue H<jats:sub>2<\/jats:sub>S content was measured by the methylene blue method. The effects of NaHS (1\u2009\u00d7\u200910<jats:sup>\u22129<\/jats:sup> to 3\u2009\u00d7\u200910<jats:sup>\u22124<\/jats:sup>\u2009M) were evaluated on carbachol (10<jats:sup>\u22125<\/jats:sup>\u2009M)\u2010induced pre\u2010contracted bladder strips, and on noradrenaline (10<jats:sup>\u22125<\/jats:sup>\u2009M)\u2010induced pre\u2010contracted prostate strips, which were pretreated with propranolol (10<jats:sup>\u22126<\/jats:sup>\u2009M). In addition, in urethane\u2010anesthetized male Wistar rats, the effects of intravesically instilled GYY4137 (10<jats:sup>\u22128<\/jats:sup>, 10<jats:sup>\u22127<\/jats:sup>, and 10<jats:sup>\u22126<\/jats:sup>\u2009M) on micturition were evaluated by cystometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MPST and CAT were detected in the bladder and prostate, CBS was only detected in the prostate, while CSE and DAO were not detected in both tissues. Immunoreactivity of these enzymes was mainly detected in the urothelium and smooth muscle layer of the bladder and in the prostate glandular epithelium. H<jats:sub>2<\/jats:sub>S was detected in both tissues. NaHS dose\u2010dependently induced relaxation of pre\u2010contracted bladder and prostate strips. Intravesically instilled GYY4137 significantly prolonged intercontraction intervals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>It is possible that H<jats:sub>2<\/jats:sub>S can function as an endogenous relaxation factor in the rat bladder and prostate.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23788","type":"journal-article","created":{"date-parts":[[2018,8,10]],"date-time":"2018-08-10T10:30:01Z","timestamp":1533897001000},"page":"2519-2526","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Possible role of hydrogen sulfide as an endogenous relaxation factor in the rat bladder and prostate"],"prefix":"10.1002","volume":"37","author":[{"given":"Suo","family":"Zou","sequence":"first","affiliation":[{"name":"Department of Pharmacology Kochi Medical School Kochi University Nankoku Kochi 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