{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:50:46Z","timestamp":1786978246323,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,9,3]],"date-time":"2018-09-03T00:00:00Z","timestamp":1535932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["MOST103\u20102314\u2010B\u2010418\u2010009"],"award-info":[{"award-number":["MOST103\u20102314\u2010B\u2010418\u2010009"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["MOST104\u20102314\u2010B\u2010418\u2010010"],"award-info":[{"award-number":["MOST104\u20102314\u2010B\u2010418\u2010010"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["MOST106\u20102320\u2010B\u2010532\u2010001"],"award-info":[{"award-number":["MOST106\u20102320\u2010B\u2010532\u2010001"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005866","name":"Far Eastern Memorial Hospital","doi-asserted-by":"publisher","award":["FEMH\u201099\u2010D\u2010006"],"award-info":[{"award-number":["FEMH\u201099\u2010D\u2010006"]}],"id":[{"id":"10.13039\/501100005866","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      We explored the therapeutic potential of intragastric administration traditional Chinese medicine\n                      <jats:italic>Glycine tomentella Hayata<\/jats:italic>\n                      (I\u2010Tiao\u2010Gung, ITG) extract and its active component Daidzin on cyclophosphamide (CYP)\u2010induced cystitis and bladder hyperactivity in rats.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Female Wistar rats were divided into control, CYP (200\u2009mg\/kg), CYP\u2009+\u2009ITG (1.17\u2009g\/kg\/day), CYP\u2009+\u2009Daidzin (12.5\u2009mg\/kg\/day), and 1 week of ITG preconditioning with CYP (ITG\u2009+\u2009CYP) groups. We determined the trans cystometrogram associated with external urethral sphincter electromyogram, and the expression of M2 and M3 muscarinic and P2\u2009\u00d7\u20092 and P2\u2009\u00d7\u20093 purinergic receptors by Western blot in these animals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      ITG extract contains 1.07% of Daidzin and 0.77% of Daidzein by high\u2010performance liquid chromatography. Daidzin was more efficient than Daidzein in scavenging H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      activity by a chemiluminescence analyzer. CYP induced higher frequency, shorter intercontraction interval, lower maximal voiding pressure, lower threshold pressure, and Phase\u20102 emptying contraction with a depressed external urethral sphincter electromyogram activity, and hemorrhagic cystitis in the bladders. The altered parameters by CYP were significantly improved in CYP\u2009+\u2009ITG, CYP\u2009+\u2009Daidzin, and ITG\u2009+\u2009CYP groups. The P2\u2009\u00d7\u20092 and P2\u2009\u00d7\u20093 expressions were significantly upregulated in CYP group, but were depressed in CYP\u2009+\u2009ITG, CYP\u2009+\u2009Daidzin, and ITG\u2009+\u2009CYP groups. The M2 expression was not significantly different among these five groups. The M3 expression was significantly upregulated in CYP group, but was significantly depressed in CYP\u2009+\u2009ITG, CYP\u2009+\u2009Daidzin, and ITG\u2009+\u2009CYP groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These data suggest that ITG extract through its active component Daidzin effectively improved CYP\u2010induced cystitis by the action of restoring Phase 2 activity and inhibiting the expressions of P2\u2009\u00d7\u20092, P2\u2009\u00d7\u20093, and M3 receptors.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/nau.23815","type":"journal-article","created":{"date-parts":[[2018,9,4]],"date-time":"2018-09-04T02:45:00Z","timestamp":1536029100000},"page":"2560-2570","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["I\u2010Tiao\u2010Gung extract through its active component daidzin improves cyclophosphamide\u2010induced bladder dysfunction in rat model"],"prefix":"10.1002","volume":"37","author":[{"given":"Kung\u2010Chieh","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Life Science, College of Science National Taiwan Normal University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing\u2010Juin","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Life Science, College of Science National Taiwan Normal University Taipei Taiwan"},{"name":"Department of Urology Cardinal Tien Hospital New Taipei City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Hsin","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Taipei City Hospital Linsen (Chinese Medicine) Branch Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1817-6106","authenticated-orcid":false,"given":"Shiu\u2010Dong","family":"Chung","sequence":"additional","affiliation":[{"name":"Division of Urology, Department of Surgery Far\u2010Eastern Memorial Hospital New Taipei City Taiwan"},{"name":"Graduate Program in Biomedical Informatics, College of Informatics Yuan\u2010Ze University Chung\u2010Li Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiang\u2010Ting","family":"Chien","sequence":"additional","affiliation":[{"name":"Department of Life Science, College of Science National Taiwan Normal University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00038.2003"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(99)00508-4"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.22609"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.5213\/inj.2012.16.1.13"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2010.09260.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(02)01786-7"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2005.04.021"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00118.2004"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706637"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-17-83"},{"key":"e_1_2_9_12_1","first-page":"205","article-title":"Effects 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