{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T23:53:30Z","timestamp":1773359610755,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,9]],"date-time":"2018-11-09T00:00:00Z","timestamp":1541721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Researcher Supporting Program of Osaka City University Graduate School of Medicine."}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate whether hyperbaric oxygen (HBO) is effective for the pathophysiological findings in an IC\/PBS\u2010like mouse model induced by intravesical hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Six\u2010week\u2010old ICR female mice (<jats:italic>N<\/jats:italic>\u2009=\u200916) were divided into four experimental groups: (1) sham control with intravesical vehicle instillation twice, and without subsequent treatment (<jats:italic>N<\/jats:italic>\u2009=\u20094); (2) H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> instillation twice, followed by HBO (100% O<jats:sub>2<\/jats:sub>, 2 ATA, 30\u2009min per session) (<jats:italic>N<\/jats:italic>\u2009=\u20094); (3) H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> instillation twice, followed by dummy hyperbaric treatment (air, 2ATA, 30\u2009min per session) (<jats:italic>N<\/jats:italic>\u2009=\u20094); and (4) H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> instillation twice, followed by no treatment (<jats:italic>N<\/jats:italic>\u2009=\u20094). Body weight, voiding frequency, tidal voiding volume, and individual bladder pain threshold using the von\u2010Frey test were measured. Whole body uptake of an inflammation\u2010specific fluorescent pan\u2010cathepsin was assessed by an in vivo imaging. Immunohistochemical staining and the mRNA expression of several biomarkers associated with chronic inflammation in resected bladders were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The HBO\u2010treated group showed significant improvement in voiding frequency, tidal voiding volume, and the individual bladder pain threshold. Moreover, HBO markedly suppressed H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced inflammation, edema, and fibrosis in bladder wall, concomitant with a significant decrease in mRNA expressions of inflammation biomarkers and a significant increase in endothelial nitric oxide synthase expression. HBO also inhibited the expression of transient receptor potential channels induced by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> instillation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that HBO contributes to elimination of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced long\u2010lasting cystitis through the repair of chronically inflamed bladder tissue and inhibition of the bladder sensory system.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23822","type":"journal-article","created":{"date-parts":[[2018,11,9]],"date-time":"2018-11-09T11:35:28Z","timestamp":1541763328000},"page":"97-106","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Hyperbaric oxygen significantly improves frequent urination, hyperalgesia, and tissue damage in a mouse long\u2010lasting cystitis model induced by an intravesical instillation of hydrogen 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