{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:02:37Z","timestamp":1790787757229,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T00:00:00Z","timestamp":1540252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK095817"],"award-info":[{"award-number":["DK095817"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Mechanosensitivity of the urinary bladder is regulated by many factors including mechano\u2010gated two\u2010pore domain (K<jats:sub>2<\/jats:sub>P, <jats:italic>KCNK<\/jats:italic>) potassium channels. TWIK\u2010related K<jats:sup>+<\/jats:sup> channel, TREK\u20101, is a predominantly expressed member of K<jats:sub>2<\/jats:sub>P channel family in the human detrusor, and its expression and function are diminished in patients with overactive lower urinary tract symptoms (LUTS). The changes in channel activity may result from spontaneously occurring gene mutations. The aim of this study was to compare single nucleotide polymorphisms (SNPs) in TREK\u20101 channel between patients with LUTS and healthy donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Six SNPs (rs370266806, rs373919966, rs758937019, rs769301539, rs772497750, and rs775158737) in two pore domains of human TREK\u20101 gene were analyzed using TaqMan SNP genotyping assay with manufacturer\u2010designed primers and allele\u2010specific probes. The screening was done in control bladders and detrusor specimens from patients with overactive LUTS. Statistical analyses were performed using R, Fisher's exact test and Hardy\u2010Weinberg Equilibrium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Six SNPs in two pore domains of the human TREK\u20101 gene were analyzed in human bladder specimens. The frequencies of rs758937019\u2010CT genotype (<jats:italic>P<\/jats:italic>\u2009=\u20090.0016) and rs758937019\u2010T allele (<jats:italic>P<\/jats:italic>\u2009=\u20090.0022) were significantly higher in the group with overactive LUTS. There was no significant association of rs775158737\u2010GA genotype and rs775158737\u2010A allele with the overactive LUTS, though they were present only in the overactive LUTS group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results provide evidence that altered expression and function of TREK\u20101 channel in patients with overactive LUTS could be due to genetic polymorphisms in the pore domains of TREK\u20101 channel (rs758937019).<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23862","type":"journal-article","created":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T12:15:39Z","timestamp":1540296939000},"page":"144-150","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Association of genetic polymorphisms in the pore domains of mechano\u2010gated TREK\u20101 channel with overactive lower urinary tract symptoms in humans"],"prefix":"10.1002","volume":"38","author":[{"given":"Balachandar","family":"Nedumaran","sequence":"first","affiliation":[{"name":"Division of Urology, Department of Surgery University of Colorado Denver Aurora Colorado"},{"name":"Division of Cardiothoracic Surgery, Department of Surgery University of Colorado Denver Aurora Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo H.","family":"Pineda","sequence":"additional","affiliation":[{"name":"Division of Urology, Department of Surgery University of Colorado Denver Aurora Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pratyaydipta","family":"Rudra","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Informatics, Colorado School of Public Health University of Colorado Denver Aurora Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanghee","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Urology University of California San Diego La Jolla California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9271-3096","authenticated-orcid":false,"given":"Anna P.","family":"Malykhina","sequence":"additional","affiliation":[{"name":"Division of Urology, Department of Surgery University of Colorado Denver Aurora 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