{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T06:07:47Z","timestamp":1760854067822,"version":"3.40.5"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,29]],"date-time":"2019-11-29T00:00:00Z","timestamp":1574985600000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2018,11,29]],"date-time":"2018-11-29T00:00:00Z","timestamp":1543449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["W81XWH\u201012\u20101\u20100565"],"award-info":[{"award-number":["W81XWH\u201012\u20101\u20100565"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["P01DK093424","R01DK088836"],"award-info":[{"award-number":["P01DK093424","R01DK088836"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>We studied the effect of herpes simplex virus (HSV) vectors\u2010based gene transfer of <jats:italic>protein phosphatase 1\u03b1 (PP1\u03b1)<\/jats:italic> on bladder hypersensitivity in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using adult female Sprague\u2010Dawley rats, non\u2010replicating HSV vectors carrying <jats:italic>PP1\u03b1<\/jats:italic> or <jats:italic>green fluorescent protein (GFP)<\/jats:italic> were injected into the bladder wall. At one week after vector inoculation, cystometry and Western blot assay were performed, whereas the other experiments were performed at 2 weeks after vector inoculation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>GFP\u2010expressing cells were identified in the bladder as well as in L6\/S1 dorsal root ganglia at 14 days. \u2009In cystometry, intercontraction intervals (ICI) after resiniferatoxin (RTx; TRPV1 agonist) irrigation was significantly reduced in the PP1\u03b1 group in comparison with the GFP group. Moreover, RTx\u2010induced freezing behavior events were observed significantly more frequently in the PP1\u03b1 group than the GFP group. The number of c\u2010Fos positive cells in the L6 spinal dorsal horn was significantly less in the PP1\u03b1 group than in the GFP group. Western blot assay revealed lower levels of phosphorylated inositol 1, 4, 5\u2010triphosphate receptor (p\u2010IP<jats:sub>3<\/jats:sub>R), and phosphorylated TRPV1 in the PP1\u03b1 compared with the GFP group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>HSV vectors\u2010mediated <jats:italic>PP1\u03b1<\/jats:italic> gene therapy may be an alternative treatment modality for cystitis\u2010related hypersensitive bladder condition at least in part via modulation of the IP<jats:sub>3<\/jats:sub>R signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23882","type":"journal-article","created":{"date-parts":[[2018,11,30]],"date-time":"2018-11-30T05:54:17Z","timestamp":1543557257000},"page":"582-590","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The effect of herpes simplex virus vector\u2010mediated gene therapy of <i>protein phosphatase 1\u03b1<\/i> on bladder overactivity and 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