{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T04:58:38Z","timestamp":1781931518622,"version":"3.54.5"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,14]],"date-time":"2018-12-14T00:00:00Z","timestamp":1544745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81500578"],"award-info":[{"award-number":["81500578"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009331","name":"Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support","doi-asserted-by":"publisher","award":["ZYLX201801"],"award-info":[{"award-number":["ZYLX201801"]}],"id":[{"id":"10.13039\/501100009331","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate the effects of injecting RNA interference (RNAi) lentiviruses targeting the muscarinic 3 (M<jats:sub>3<\/jats:sub>) receptor gene into the bladder wall on bladder activity in rats with spinal cord injury (SCI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Four M<jats:sub>3<\/jats:sub> RNAi lentiviruses were constructed and used to infect primary cultured bladder smooth muscle cells (BSMCs). Western blotting and quantitative reverse transcription polymerase chain reaction (qRT\u2010PCR) were performed to determine the optimal RNAi lentivirus with the highest interference efficiency. Female Wistar rats were subjected spinal cord transection at T9\u201010 and randomly divided into three groups (<jats:italic>n<\/jats:italic>\u2009=\u20098), namely, blank control, negative control, and experimental groups, and injected into the bladder wall with saline, negative control shRNA, and M<jats:sub>3<\/jats:sub> RNAi lentiviruses, respectively, 1 week after spinal cord transection. The normal rats were used as normal control group. Urodynamic parameters and bladder tissues were evaluated in the different groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>An M<jats:sub>3<\/jats:sub> RNAi lentivirus with the highest interference efficiency (78.9%) was constructed and identified. Three weeks after injecting M<jats:sub>3<\/jats:sub> RNAi lentiviruses into the bladder wall, Western blotting and qRT\u2010PCR showed that the M<jats:sub>3<\/jats:sub> receptor was significantly downregulated in the experimental group. Cystometric evaluation suggested that downregulating M<jats:sub>3<\/jats:sub> receptor expression could substantially decrease basal pressure, residual volume, and non\u2010voiding contraction number, increase intercontraction interval, and significantly improve bladder compliance in rats with SCI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Injecting RNAi lentiviruses targeting the M<jats:sub>3<\/jats:sub> receptor gene into the bladder wall could effectively inhibit neurogenic detrusor overactivity (NDO) due to SCI. Thus, this approach may be a potential treatment for NDO in SCI.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23894","type":"journal-article","created":{"date-parts":[[2018,12,14]],"date-time":"2018-12-14T10:41:25Z","timestamp":1544784085000},"page":"615-624","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Injecting RNA interference lentiviruses targeting the muscarinic 3 receptor gene into the bladder wall inhibits neurogenic detrusor overactivity in rats with spinal cord injury"],"prefix":"10.1002","volume":"38","author":[{"given":"Zhenhua","family":"Shang","sequence":"first","affiliation":[{"name":"Department of Urology Xuanwu Hospital Capital Medical University Xuanwu District Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunsong","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Urology Xuanwu Hospital Capital Medical University Xuanwu District 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