{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T13:31:12Z","timestamp":1775482272472,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,3,14]],"date-time":"2019-03-14T00:00:00Z","timestamp":1552521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>The main goal of our study was to investigate whether blebbistatin would prevent the cyclophosphamide (CYP)\u2010induced changes in cystometric and inflammatory parameters indicating the development of bladder inflammation and bladder overactivity. As the nature of CYP\u2010induced urotoxicity is inflammatory, we assume that agents presenting an anti\u2010inflammatory potential, such as blebbistatin,\u00a0are worth special attention.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The experiments were carried out in female Wistar rats. Surgical procedures, cystometric investigations, measurements of bladder edema and urothelium thickness as well as biochemical analyses were performed according to the published literature.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>As expected, an acute administration of CYP (200\u2009mg\/kg, intraperitoneally) induced changes in the cystometric parameters and the levels of the tested biomarkers (ie, interleukin 1\u2010\u03b2, interleukin 6, interleukin 10, tumor necrosis factor\u2010\u03b1, nerve growth factor, brain\u2010derived neurotrophic factor, heparin\u2010binding epidermal growth factor\u2010like growth factor, insulin\u2010like growth factor\u2010binding protein 3, C\u2010X\u2010C motif chemokine 10, orosomucoid\u20101, Tamm\u2010Horsfall protein, hemopexin, and occludin), indicating the development of bladder overactivity and bladder inflammation, respectively. These changes were accompanied by bladder edema and increased urothelium thickness. Intravesical infusion of blebbistatin for 7 days (125\u2009nmol\/day) prevented all symptoms of the CYP\u2010induced urotoxicity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Blebbistatin might be a promising novel agent for the treatment of bladder dysfunctions, like CYP\u2010induced hemorrhage cystitis or bladder overactivity, since it diminished the increased urinary bladder levels of proinflammatory markers and normalized the concentrations of the anti\u2010inflammatory ones. This effect was accompanied by amelioration of bladder edema and permeability, and normalization of both urothelium thickness and values of the cystometric parameters.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.23973","type":"journal-article","created":{"date-parts":[[2019,3,14]],"date-time":"2019-03-14T14:06:59Z","timestamp":1552572419000},"page":"1044-1052","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Intravesical administration of blebbistatin prevents cyclophosphamide\u2010induced toxicity of the urinary bladder in female Wistar rats"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5772-0573","authenticated-orcid":false,"given":"Andrzej","family":"Wr\u00f3bel","sequence":"first","affiliation":[{"name":"Second Department of Gynaecology Medical University of Lublin Lublin 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