{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T06:08:36Z","timestamp":1790489316776,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,8,11]],"date-time":"2019-08-11T00:00:00Z","timestamp":1565481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To identify differences in the vaginal microbiomes of women after transvaginal mesh (TVM) surgery for pelvic organ prolapse with and without mesh\u2010associated complications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients with complications were eligible as cases, patients without as controls. DNA was isolated and the V1\u20102 region of the 16S ribosomal RNA gene was amplified and sequenced. Overall richness was quantified using Chao1. Overall diversity was expressed as Shannon diversity and screened for group differences using analysis of variance. Multivariate differences among groups were evaluated with functions from <jats:italic>R<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We recruited 14 patients after mesh exposure, 5 after contraction, and 21 as controls. The average number of operational taxonomic unit was 74.79 (SD\u2009\u00b1\u200963.91) for controls, 57.13 (SD\u2009\u00b1\u200958.74) after exposures, and 92.42 (SD\u2009\u00b1\u200950.01) after contractions. Total 89.6% of bacteria in controls, 86.4% in previous exposures, and 81.3% in contractions were classified as either <jats:italic>Firmicutes, Proteobacteria<\/jats:italic>, or <jats:italic>Actinobacteria<\/jats:italic> (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001). <jats:italic>Veillonella<\/jats:italic> spp. was more abundant in patients after contraction (<jats:italic>P<\/jats:italic>\u2009=\u2009.045). The individual microbiomes varied, and we did not detect any significant differences in richness but a trend towards higher diversity with complications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The presence of <jats:italic>Veillonella<\/jats:italic> spp. could be associated with mesh contraction. Our study did not identify vaginal microbiotic dysbiosis as a factor associated with exposure. Larger cohort studies would be needed to distinguish the vaginal microbiome of women predisposed to mesh\u2010related complications for targeted phenotyping of patients who could benefit from TVM surgery.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.24129","type":"journal-article","created":{"date-parts":[[2019,8,12]],"date-time":"2019-08-12T05:24:39Z","timestamp":1565587479000},"page":"2255-2263","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Abnormal vaginal microbiome associated with vaginal mesh complications"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2102-9254","authenticated-orcid":false,"given":"Nikolaus","family":"Veit\u2010Rubin","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4352-4782","authenticated-orcid":false,"given":"Renaud","family":"De Tayrac","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Caremeau University Hospital  N\u00eemes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rufus","family":"Cartwright","sequence":"additional","affiliation":[{"name":"Department of Urogynaecology Oxford University Hospitals  Oxford United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa","family":"Franklin\u2010Revill","sequence":"additional","affiliation":[{"name":"Department of Urogynaecology, St. Mary's Hospital Imperial College London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Warembourg","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics CHU La Croix\u2010Rousse University Hospital Lyon  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Dunyach\u2010Remy","sequence":"additional","affiliation":[{"name":"Department of Microbiology, CHU Caremeau University Hospital Montpellier University  N\u00eemes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9484-0304","authenticated-orcid":false,"given":"Jean\u2010Philippe","family":"Lavigne","sequence":"additional","affiliation":[{"name":"Department of Microbiology, CHU Caremeau University Hospital Montpellier University  N\u00eemes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4775-7495","authenticated-orcid":false,"given":"Vik","family":"Khullar","sequence":"additional","affiliation":[{"name":"Department of Urogynaecology, St. Mary's Hospital Imperial College London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,11]]},"reference":[{"key":"e_1_2_6_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-007-0459-9"},{"key":"e_1_2_6_1_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/OGX.0b013e318166fb44"},{"key":"e_1_2_6_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2011.03.027"},{"key":"e_1_2_6_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.21036"},{"key":"e_1_2_6_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-009-1021-8"},{"key":"e_1_2_6_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2008.07.049"},{"key":"e_1_2_6_1_8_1","unstructured":"FDA takes action to protect women's health orders manufacturers of surgical mesh intended for transvaginal repair of pelvic organ prolapse to stop selling all devices [press release].2019."},{"key":"e_1_2_6_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)31596-3"},{"key":"e_1_2_6_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgyn.2013.03.012"},{"key":"e_1_2_6_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2018.01.044"},{"key":"e_1_2_6_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2008.01.048"},{"key":"e_1_2_6_1_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-011-1405-4"},{"key":"e_1_2_6_1_14_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.12-220806"},{"key":"e_1_2_6_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201100359"},{"key":"e_1_2_6_1_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-S8-S17"},{"key":"e_1_2_6_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3129"},{"key":"e_1_2_6_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2014.08.006"},{"key":"e_1_2_6_1_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aag1026"},{"key":"e_1_2_6_1_20_1","unstructured":"\u00a0R Development Core Team.R: a language and environment for statistical computing. 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