{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:14:38Z","timestamp":1791346478052,"version":"4.3.1"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T00:00:00Z","timestamp":1566864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["1R01DK105097"],"award-info":[{"award-number":["1R01DK105097"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["R56DK105097"],"award-info":[{"award-number":["R56DK105097"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate the possibility and mechanism of microenergy acoustic pulses (MAP) for activating tissue resident stem\/progenitor cells within pelvic and urethral muscle and possible mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The female Zucker Lean and Zucker Fatty rats were randomly divided into four groups: ZL control, ZLMAP, ZF control, and ZFMAP. MAP was applied at 0.033\u2009mJ\/mm<jats:sup>2<\/jats:sup>, 3\u2009Hz for 500 pulses, and the urethra and pelvic floor muscles of each rat was then harvested for cell isolation and flow cytometry assay. Freshly isolated cells were analyzed by flow cytometry for Pax\u20107, Int\u20107\u03b1, H3P, and EdU expression. Meanwhile, pelvic floor muscle\u2010derived stem cells (MDSCs) were harvested through magnetic\u2010activated cell sorting, MAP was then applied to MDSCs to assess the mechanism of stem cell activation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Obesity reduced EdU\u2010label\u2010retaining cells and satellite cells in both pelvic floor muscle and urethra, while MAP activated those cells and enhanced cell proliferation, which promoted regeneration of striated muscle cells of the pelvic floor and urethral sphincter. Activation of focal adhesion kinase (FAK)\/AMP\u2010activated protein kinase (AMPK) \/Wnt\/\u03b2\u2010catenin signaling pathways by MAP is the potential mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>MAP treatment activated tissue resident stem cells within pelvic floor and urethral muscle in situ via activating FAK\u2010AMPK and Wnt\/\u03b2\u2010catenin signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.24152","type":"journal-article","created":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T08:10:14Z","timestamp":1566893414000},"page":"2140-2150","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The effects of microenergy acoustic pulses on animal model of obesity\u2010associated stress urinary incontinence. Part 2: In situ activation of pelvic floor and urethral striated muscle progenitor cells"],"prefix":"10.1002","volume":"38","author":[{"given":"Ning","family":"Kang","sequence":"first","affiliation":[{"name":"Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine University of California  San Francisco California"},{"name":"Department of Urology, Chaoyang Hospital Beijing Captial Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongyi","family":"Peng","sequence":"additional","affiliation":[{"name":"Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine University of California  San Francisco California"},{"name":"Department of Urology Third Xiangya Hospital of Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bohan","family":"Wang","sequence":"additional","affiliation":[{"name":"Knuppe Molecular Urology Laboratory, Department of 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