{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T07:07:05Z","timestamp":1776928025692,"version":"3.51.2"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,3]],"date-time":"2020-10-03T00:00:00Z","timestamp":1601683200000},"content-version":"am","delay-in-days":366,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2019,10,3]],"date-time":"2019-10-03T00:00:00Z","timestamp":1570060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["W81XWH\u201017\u20101\u20100403"],"award-info":[{"award-number":["W81XWH\u201017\u20101\u20100403"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["NIH P01 DK093424"],"award-info":[{"award-number":["NIH P01 DK093424"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To investigate the role of p38 MAP kinase in lower urinary tract dysfunction in mice with spinal cord injury (SCI).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Cystometry and external urethral sphincter\u2010electromyography were performed under an awake condition in 4\u2010week SCI female mice. Two weeks after SCI, a catheter connected to an osmotic pump filled with a p38 mitogen\u2010activated protein kinase (MAPK) inhibitor or artificial cerebrospinal fluid (CSF) was implanted into the intrathecal space of L6\u2010S1 spinal cord for continuous intrathecal instillation at infusion rate of 0.51\u2009\u03bcL\/h for 2\u2009weeks before the urodynamic study. L6 dorsal root ganglia were then removed from CSF and p38 MAPK inhibitor\u2010treated SCI mice as well as from CSF\u2010treated normal (spinal intact) mice to evaluate the levels of transient receptor potential cation channel subfamily V member 1 (TRPV1), tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), and inducible nitric oxide synthase (iNOS) transcripts by real\u2010time polymerase chain reaction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In p38 MAPK inhibitor\u2010treated SCI mice, nonvoiding contractions during bladder filling, bladder capacity, and post\u2010void residual volume were significantly reduced while micturition pressure and voiding efficiency were significantly increased in comparison to these measurements in CSF\u2010treated SCI mice. The expression of TRPV1, TNF\u2010\u03b1, and iNOS messenger RNA was increased in SCI mice compared with expression in spinal intact mice and significantly decreased after p38 MAPK inhibitor treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The p38 MAPK signaling pathway in bladder sensory neurons or in the spinal cord plays an important role in storage and voiding problems such as detrusor overactivity and inefficient voiding after SCI.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/nau.24170","type":"journal-article","created":{"date-parts":[[2019,10,3]],"date-time":"2019-10-03T06:14:01Z","timestamp":1570083241000},"page":"108-115","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Role of p38 MAP kinase signaling pathways in storage and voiding dysfunction in mice with spinal cord injury"],"prefix":"10.1002","volume":"39","author":[{"given":"Nobutaka","family":"Shimizu","sequence":"first","affiliation":[{"name":"Department of Urology University of Pittsburgh  Pittsburgh Pennsylvania"},{"name":"Department of Urology, Faculty of Medicine Kindai University  Osaka\u2010Sayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9587-6798","authenticated-orcid":false,"given":"Naoki","family":"Wada","sequence":"additional","affiliation":[{"name":"Department of Urology University of Pittsburgh  Pittsburgh Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1555-2284","authenticated-orcid":false,"given":"Takahiro","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Urology University of Pittsburgh  Pittsburgh Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahisa","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Urology University of Pittsburgh  Pittsburgh Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Kurobe","sequence":"additional","affiliation":[{"name":"Department of Urology University of Pittsburgh  Pittsburgh Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony J.","family":"Kanai","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Pittsburgh  Pittsburgh Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William C.","family":"de Groat","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology University of Pittsburgh  Pittsburgh 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