{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T07:56:44Z","timestamp":1793692604080,"version":"4.5.1"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,3,9]],"date-time":"2020-03-09T00:00:00Z","timestamp":1583712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ftirma Kurumu","doi-asserted-by":"publisher","award":["216S813"],"award-info":[{"award-number":["216S813"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To evaluate the impacts of hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) donor, sodium hydrogen sulfide (NaHS), and phosphodiesterase type\u20105 inhibitor (PDE5i), tadalafil per se and their combination treatment on partial bladder outlet obstruction (PBOO)\u2010induced erectile dysfunction (ED).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sprague\u2010Dawley rats were equally divided into five groups: (a) sham\u2010operated control; (b) PBOO; (c) PBOO\u2010treated with NaHS (5.6\u2009mg\/kg\/day, ip); (d) PBOO\u2010treated with tadalafil (2\u2009mg\/kg\/day, oral); and (e) PBOO\u2010treated with combination of NaHS and tadalafil. The obstruction was created by urethral ligation for 6 weeks. In vivo erectile responses, in vitro relaxant and contractile responses in penile tissue as well as protein expression of nitric oxide synthases (NOS), H<jats:sub>2<\/jats:sub>S synthesis enzymes, oxidative stress, hypoxia, fibrosis markers, and the smooth muscle\/collagen ratio and apoptosis were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Combined treatment entirely returned increased bladder mass, reduced erectile responses, relaxation responses to acetylcholine, and electrical field stimulation in obstructed rats, while partial amelioration was observed after mono\u2010treatment. Decreased neuronal NOS and 3\u2010mercaptopiruvate transferase enzyme expressions in penile tissue from obstructed rats were also entirely restored by the combined treatment. Mono\u2010treatment partially improved increased hypoxia, oxidative stress, fibrosis markers, decreased smooth muscle mass, and H<jats:sub>2<\/jats:sub>S levels, while combined therapy completely recovered.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The combination therapy with H<jats:sub>2<\/jats:sub>S donor and PDE5i had positive effects on erectile responses through the improvement of ischemia\u2010induced morphological and functional penile alterations in obstruction. H<jats:sub>2<\/jats:sub>S and NO may likely play a synergistic role in the regulation of erectile function and have constructive effects on clinical outcomes in male patients with ED and benign prostatic hyperplasia\/lower urinary tract symptoms.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.24333","type":"journal-article","created":{"date-parts":[[2020,3,9]],"date-time":"2020-03-09T15:59:11Z","timestamp":1583769551000},"page":"1087-1097","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Evaluation of combined therapeutic effects of hydrogen sulfide donor sodium hydrogen sulfide and phosphodiesterase type\u20105 inhibitor tadalafil on erectile dysfunction in a partially bladder outlet obstructed rat 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