{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T15:43:16Z","timestamp":1773416596868,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T00:00:00Z","timestamp":1592179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japanese Society for the Promotion of Science","doi-asserted-by":"crossref","award":["15K10617"],"award-info":[{"award-number":["15K10617"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>We develop a novel rabbit urinary diversion model of bladder defunctionalization due to bladder anuria followed by refunctionalization due to urine reperfusion to investigate the molecular biological background. To validate the results, we used reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) to analyze human specimens from defunctionalized bladders in patients receiving dialysis before kidney transplantation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Female rabbits were divided into three groups: control, defunctionalized, and refunctionalized. The bilateral ureters were anastomosed to vagina in the defunctionalized and refunctionalized groups at 0 weeks. In the refunctionalized group, the unilateral ureter was reanastomosed to the bladder at 8 weeks.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The capacity and compliance of the rabbit bladder in the refunctionalized group were significantly lower than those in the control group at 8 weeks and higher than those in the defunctionalized group at 14 weeks. The significant downregulation of\n                      <jats:italic>IGFBP2<\/jats:italic>\n                      ,\n                      <jats:italic>UPK1B<\/jats:italic>\n                      , and\n                      <jats:italic>CST6<\/jats:italic>\n                      in the defunctionalized group compared with that in the control groups, and the significant downregulation of\n                      <jats:italic>AGTR2<\/jats:italic>\n                      in the refunctionalized group compared with that in the defunctionalized group in the rabbit bladder\u2010muscle DNA microarray were validated by RT\u2010PCR. Human bladder muscle indicated significant downregulation of\n                      <jats:italic>UPK1B<\/jats:italic>\n                      and\n                      <jats:italic>CST6<\/jats:italic>\n                      and significant downregulation of\n                      <jats:italic>IGFBP2<\/jats:italic>\n                      in the defunctionalized group, which is consistent with both rabbit bladder\u2010muscle DNA microarray and rabbit bladder RT\u2010PCR results.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The present study using novel model of bladder defunctionalization followed by refunctionalization indicated the consistent downregulation of\n                      <jats:italic>UPK1B<\/jats:italic>\n                      and\n                      <jats:italic>CST6<\/jats:italic>\n                      in muscle and the consistent downregulation of\n                      <jats:italic>IGFBP2<\/jats:italic>\n                      in mucosa in process of bladder defunctionalization, which was validated by human specimens.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/nau.24427","type":"journal-article","created":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T13:35:21Z","timestamp":1592228121000},"page":"1653-1666","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Candidate genes involved in the defunctionalization and refunctionalization of the urinary bladder induced by bladder anuria and reperfusion"],"prefix":"10.1002","volume":"39","author":[{"given":"Yusuke","family":"Kisou","sequence":"first","affiliation":[{"name":"Department of Urology Akita University Graduate School of Medicine  Akita 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