{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:10:28Z","timestamp":1792667428767,"version":"4.5.0"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T00:00:00Z","timestamp":1591920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17K11187"],"award-info":[{"award-number":["17K11187"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>As PDGFR\u03b1 (+) cells appear not to suppress the excitability of detrusor smooth muscle by generating SK3\u2010dependent hyperpolarising as proposed in the gastrointestinal tract, we further explored the functional roles of PDGFR\u03b1 (+) cells in regulating the spontaneous activity of urogenital tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using PDGFR\u03b1\u2010eGFP mice, intracellular Ca<jats:sup>2+<\/jats:sup> signaling in PDGFR\u03b1 (+) cells of the bladder lamina propria, renal pelvis, and seminal vesicle were visualized using Cal\u2010590 fluorescence. The distribution and SK3 expression of PDGFR\u03b1 (+) cells were also examined by immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the bladder lamina propria, SK3 (\u2212) PDGFR\u03b1 (+) cells exhibited spontaneous Ca<jats:sup>2+<\/jats:sup> transients and responded to stimulation of P2Y1 purinoceptors with MRS2365 (100\u2009nM) or adenosine diphosphate (ADP) (100\u2009\u03bcM) by developing Ca<jats:sup>2+<\/jats:sup> transients. In the proximal renal pelvis, PDGFR\u03b1 (+) cells were distributed in the mucosal, muscular and serosal layers but did not express SK3 immunoreactivity. PDGFR\u03b1 (+) cells in the musculature resembling atypical smooth muscle cells generated spontaneous Ca<jats:sup>2+<\/jats:sup> transients that were partially suppressed upon P2Y1\u2010stimulation, while vigorously responding to human angiotensin II (100\u2009nM). In the seminal vesicle, PDGFR\u03b1 (+) cells in the musculature but not mucosa expressed SK3 immunoreactivity. In the mucosa, the P2Y1 stimulation evoked Ca<jats:sup>2+<\/jats:sup> transients in both PDGFR\u03b1 (+) cells and PDGFR\u03b1 (\u2212) cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PDGFR\u03b1 (+) cells in spontaneously active urogenital tissues display heterogeneity in terms of their SK3 expression and P2Y1\u2010induced Ca<jats:sup>2+<\/jats:sup> responses. Muscular PDGFR\u03b1 (+) cells in the renal pelvis and mucosal PDGFR\u03b1 (+) cells in the seminal vesicle may generate depolarizing signals to drive smooth muscle cells.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.24431","type":"journal-article","created":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T21:14:06Z","timestamp":1591996446000},"page":"1667-1678","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Functional heterogeneity of PDGFR\u03b1 (+) cells in spontaneously active urogenital tissues"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3877-4349","authenticated-orcid":false,"given":"Hikaru","family":"Hashitani","sequence":"first","affiliation":[{"name":"Department of Cell Physiology Nagoya City University Graduate School of Medical Sciences Nagoya 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